Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS

Falk Schneider, Mathias P. Clausen, Dominic Waithe, Thomas Koller, Gunes Ozhan, Christian Eggeling, View ORCID ProfileErdinc Sezgin
doi: https://doi.org/10.1101/076109
Falk Schneider
aMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, OX39DS, Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mathias P. Clausen
aMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, OX39DS, Oxford, United Kingdom
bMEMPHYS – Center for Biomembrane Physics, Department of Physics, Chemistry, and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dominic Waithe
cWolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford,OX39DS, Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Koller
aMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, OX39DS, Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gunes Ozhan
dIzmir International Biomedicine and Genome Institute (iBG-izmir), Dokuz Eylul University, Inciralti-Balcova, 35340 Izmir, Turkey;
eDepartment of Medical Biology and Genetics, Dokuz Eylul University Medical School, Inciralti-Balcova, 35340 Izmir, Turkey.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christian Eggeling
aMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, OX39DS, Oxford, United Kingdom
cWolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford,OX39DS, Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: erdinc.sezgin@rdm.ox.ac.uk christian.eggeling@rdm.ox.ac.uk
Erdinc Sezgin
aMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, OX39DS, Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Erdinc Sezgin
  • For correspondence: erdinc.sezgin@rdm.ox.ac.uk christian.eggeling@rdm.ox.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Diffusion and interaction dynamics of molecules at the plasma membrane play an important role in cellular signalling. These have been suggested to be strongly associated with the actin cytoskeleton. Here, we utilise super-resolution STED microscopy combined with fluorescence correlation spectroscopy (STED-FCS) to access the sub-diffraction diffusion regime of different fluorescent lipid analogues and GPI-anchored proteins (GPI-APs) in the cellular plasma membrane, and compare it to the diffusion regime of these molecules in cell-derived actin-free giant plasma membrane vesicles (GPMVs). We show that phospholipids and sphingomyelin, which undergo hindered diffusion in the live cell membrane, diffuse freely in the GPMVs. In contrast to sphingomyelin, which is transiently trapped on molecular-scale complexes in intact cells, diffusion of the ganglioside lipid GM1 suggests transient incorporation into nanodomains, which is less influenced by the actin cortex. Finally, our data on GPI-APs indicate two molecular pools in living cells, one pool showing high mobility with trapped and compartmentalized diffusion, and the other forming immobile clusters both of which disappear in GPMVs. Our data underlines the crucial role of the actin cortex in maintaining hindered diffusion modes of most but not all membrane molecules.

GPMVs
giant plasma membrane vesicles
GUVs
giant unilamellar vesicles
GPI-AP
Glycophosphatidylinositol anchored protein
PL
phospholipid
STED
Stimulated emission depletion
FCS
fluorescence correlation spectroscopy
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted September 19, 2016.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS
Falk Schneider, Mathias P. Clausen, Dominic Waithe, Thomas Koller, Gunes Ozhan, Christian Eggeling, Erdinc Sezgin
bioRxiv 076109; doi: https://doi.org/10.1101/076109
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS
Falk Schneider, Mathias P. Clausen, Dominic Waithe, Thomas Koller, Gunes Ozhan, Christian Eggeling, Erdinc Sezgin
bioRxiv 076109; doi: https://doi.org/10.1101/076109

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3503)
  • Biochemistry (7346)
  • Bioengineering (5321)
  • Bioinformatics (20259)
  • Biophysics (10013)
  • Cancer Biology (7742)
  • Cell Biology (11298)
  • Clinical Trials (138)
  • Developmental Biology (6437)
  • Ecology (9950)
  • Epidemiology (2065)
  • Evolutionary Biology (13318)
  • Genetics (9360)
  • Genomics (12581)
  • Immunology (7700)
  • Microbiology (19016)
  • Molecular Biology (7439)
  • Neuroscience (41024)
  • Paleontology (300)
  • Pathology (1228)
  • Pharmacology and Toxicology (2135)
  • Physiology (3157)
  • Plant Biology (6860)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1895)
  • Systems Biology (5311)
  • Zoology (1089)