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An odor delivery system for arbitrary time-varying patterns of odors, mixtures and concentrations

Priyanka Gupta, Dinu F Albeanu, Upinder S Bhalla
doi: https://doi.org/10.1101/077875
Priyanka Gupta
1National Center for Biological Sciences, Bangalore, Karnataka 560065, India
2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Dinu F Albeanu
2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
3Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
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Upinder S Bhalla
1National Center for Biological Sciences, Bangalore, Karnataka 560065, India
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Abstract

Odor stimuli in the natural environment are intermittent and the concentration of any given odor fluctuates rapidly over time. Further, even in the simplest scenario, the olfactory sensors receive uncorrelated, intermittent inputs in the form of odor plumes arising from several odor sources in the local environment. However, typically used odor stimuli under laboratory settings are restricted to long-duration (~seconds), single pulse of one odor at a time that are rarely encountered in nature. This inadequate choice of odor stimuli is due to the dearth of affordable odor delivery systems that can generate plume-like, naturalistic stimuli with high reproducibility such as to allow for repeat measurements under laboratory conditions. We thus developed an odor delivery system that generates arbitrary time-varying patterns of individual odors and ternary mixtures at time scales of ~20 Hz. Here, we provide a detailed description of the construction and output characterization of our odor delivery system.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted September 29, 2016.
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An odor delivery system for arbitrary time-varying patterns of odors, mixtures and concentrations
Priyanka Gupta, Dinu F Albeanu, Upinder S Bhalla
bioRxiv 077875; doi: https://doi.org/10.1101/077875
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An odor delivery system for arbitrary time-varying patterns of odors, mixtures and concentrations
Priyanka Gupta, Dinu F Albeanu, Upinder S Bhalla
bioRxiv 077875; doi: https://doi.org/10.1101/077875

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