Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing

Robert C. Edgar
doi: https://doi.org/10.1101/081257
Robert C. Edgar
Independent Investigator Tiburon, California, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: robert@drive5.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for investigating microbial populations. In such experiments, sequence errors caused by PCR and sequencing are difficult to distinguish from true biological variation. I describe UNOISE2, an updated version of the UNOISE algorithm for denoising (error-correcting) Illumina amplicon reads and show that it has comparable or better accuracy than DADA2.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted October 15, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing
Robert C. Edgar
bioRxiv 081257; doi: https://doi.org/10.1101/081257
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing
Robert C. Edgar
bioRxiv 081257; doi: https://doi.org/10.1101/081257

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Bioinformatics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4672)
  • Biochemistry (10333)
  • Bioengineering (7654)
  • Bioinformatics (26281)
  • Biophysics (13497)
  • Cancer Biology (10663)
  • Cell Biology (15392)
  • Clinical Trials (138)
  • Developmental Biology (8485)
  • Ecology (12802)
  • Epidemiology (2067)
  • Evolutionary Biology (16818)
  • Genetics (11380)
  • Genomics (15453)
  • Immunology (10592)
  • Microbiology (25151)
  • Molecular Biology (10193)
  • Neuroscience (54373)
  • Paleontology (399)
  • Pathology (1663)
  • Pharmacology and Toxicology (2889)
  • Physiology (4332)
  • Plant Biology (9223)
  • Scientific Communication and Education (1585)
  • Synthetic Biology (2552)
  • Systems Biology (6769)
  • Zoology (1459)