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Potent optogenetic inhibition of behavior with anion channelrhodopsins

View ORCID ProfileFarhan Mohammad, View ORCID ProfileJames C. Stewart, View ORCID ProfileStanislav Ott, View ORCID ProfileKatarina Chlebikova, View ORCID ProfileJia Yi Chua, View ORCID ProfileTong-Wey Koh, View ORCID ProfileJoses Ho, View ORCID ProfileAdam Claridge-Chang
doi: https://doi.org/10.1101/082255
Farhan Mohammad
1Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore 138673
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James C. Stewart
2Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673
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Stanislav Ott
1Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore 138673
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Katarina Chlebikova
1Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore 138673
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  • ORCID record for Katarina Chlebikova
Jia Yi Chua
1Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore 138673
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Tong-Wey Koh
4Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604
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Joses Ho
2Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673
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Adam Claridge-Chang
1Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore 138673
2Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673
3Department of Physiology, National University of Singapore, Singapore 138673
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  • For correspondence: claridge-chang.adam@duke-nus.edu.sg
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Abstract

Optogenetics employs light exposure to manipulate physiology in genetically modified organisms. There are abundant tools for optogenetic excitation of neuronal activity, but the limitations of current activity photo-inhibitors present an obstacle to demonstrating the necessity of specific neuronal circuits. Here we show that anion channelrhodopsins can be used to specifically and rapidly inhibit a range of systems involved in Drosophila locomotion, wing expansion, memory retrieval and gustation, demonstrating their broad utility to the circuit analysis of behavior.

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Posted October 20, 2016.
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Potent optogenetic inhibition of behavior with anion channelrhodopsins
Farhan Mohammad, James C. Stewart, Stanislav Ott, Katarina Chlebikova, Jia Yi Chua, Tong-Wey Koh, Joses Ho, Adam Claridge-Chang
bioRxiv 082255; doi: https://doi.org/10.1101/082255
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Potent optogenetic inhibition of behavior with anion channelrhodopsins
Farhan Mohammad, James C. Stewart, Stanislav Ott, Katarina Chlebikova, Jia Yi Chua, Tong-Wey Koh, Joses Ho, Adam Claridge-Chang
bioRxiv 082255; doi: https://doi.org/10.1101/082255

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