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CENP-F couples cargo to growing and shortening microtubule ends

Gil Kanfer, Martin Peterka, Vladimir K. Arzhanik, Alexei L. Drobyshev, Fazly I. Ataullakhanov, Vladimir A. Volkov, Benoît Kornmann
doi: https://doi.org/10.1101/083618
Gil Kanfer
1Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
2present address: Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
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Martin Peterka
1Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
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Vladimir K. Arzhanik
3Department of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia
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Alexei L. Drobyshev
4Center for theoretical problems of physicochemical pharmacology, Russian Academy of Sciences, Moscow, Russia
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Fazly I. Ataullakhanov
4Center for theoretical problems of physicochemical pharmacology, Russian Academy of Sciences, Moscow, Russia
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Vladimir A. Volkov
4Center for theoretical problems of physicochemical pharmacology, Russian Academy of Sciences, Moscow, Russia
5present address: Department of Bionanoscience, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands
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Benoît Kornmann
1Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
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Abstract

Dynamic microtubule ends exert pulling and pushing forces on intracellular membranes and organelles. However, the mechanical linkage of microtubule tips to their cargoes is poorly understood. CENP-F is a non-motor microtubule-binding protein that participates in microtubule binding at kinetochores and in the mitotic redistribution of the mitochondrial network. CENP-F-driven mitochondrial transport is linked to growing microtubule tips, but the underlying molecular mechanisms are unknown. Here we show that CENP-F tracks growing microtubule ends in living cells. In vitro reconstitution demonstrates that microtubule tips can transport mitochondria and CENP-F-coated artificial cargoes over micrometer-long distances, during both growing and shrinking phases. Based on these and previous observations, we suggest that CENP-F might act as a transporter of mitochondria and other cellular cargoes by attaching them to dynamic microtubule ends.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 26, 2016.
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CENP-F couples cargo to growing and shortening microtubule ends
Gil Kanfer, Martin Peterka, Vladimir K. Arzhanik, Alexei L. Drobyshev, Fazly I. Ataullakhanov, Vladimir A. Volkov, Benoît Kornmann
bioRxiv 083618; doi: https://doi.org/10.1101/083618
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CENP-F couples cargo to growing and shortening microtubule ends
Gil Kanfer, Martin Peterka, Vladimir K. Arzhanik, Alexei L. Drobyshev, Fazly I. Ataullakhanov, Vladimir A. Volkov, Benoît Kornmann
bioRxiv 083618; doi: https://doi.org/10.1101/083618

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