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Cell wall remodeling drives engulfment during Bacillus subtilis sporulation

Nikola Ojkic, Javier López-Garrido, Kit Pogliano, Robert G. Endres
doi: https://doi.org/10.1101/087858
Nikola Ojkic
1Department of Life Sciences, Imperial College London, London, United Kingdom
2Centre for Integrative Systems Biology and Bioinformatics, Imperial College London, London, UK
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Javier López-Garrido
3Division of Biological Sciences, University of California at San Diego, La Jolla, California, USA
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Kit Pogliano
3Division of Biological Sciences, University of California at San Diego, La Jolla, California, USA
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  • For correspondence: kpogliano@ucsd.edu r.endres@imperial.ac.uk
Robert G. Endres
1Department of Life Sciences, Imperial College London, London, United Kingdom
2Centre for Integrative Systems Biology and Bioinformatics, Imperial College London, London, UK
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  • For correspondence: kpogliano@ucsd.edu r.endres@imperial.ac.uk
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Abstract

When starved, the Gram-positive bacterium Bacillus subtilis forms durable spores for survival. Sporulation initiates with an asymmetric cell division, creating a large mother cell and a small forespore. Subsequently, the mother cell membrane engulfs the forespore in a phagocytosis-like process. However, the force generation mechanism for forward membrane movement remains unknown. Here, we show that membrane migration is driven by cell wall remodeling at the leading edge of the engulfing membrane, with peptidoglycan synthesis and degradation mediated by penicillin binding proteins in the forespore and a cell wall degradation protein complex in the mother cell. We propose a simple model for engulfment in which the junction between the septum and the lateral cell wall moves around the forespore by a mechanism resembling the ‘template model’. Hence, we establish a biophysical mechanism for the creation of a force for engulfment based on the coordination between cell wall synthesis and degradation.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 15, 2016.
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Cell wall remodeling drives engulfment during Bacillus subtilis sporulation
Nikola Ojkic, Javier López-Garrido, Kit Pogliano, Robert G. Endres
bioRxiv 087858; doi: https://doi.org/10.1101/087858
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Cell wall remodeling drives engulfment during Bacillus subtilis sporulation
Nikola Ojkic, Javier López-Garrido, Kit Pogliano, Robert G. Endres
bioRxiv 087858; doi: https://doi.org/10.1101/087858

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