Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis

Hui Dong, Jack Dumenil, Fu-Hao Lu, Li Na, Hannes Vanhaeren, Christin Naumann, Maria Klecker, Rachel Prior, Caroline Smith, Neil McKenzie, Gerhard Saalbach, Liangliang Chen, Tian Xia, Nathalie Gonzalez, Mathilde Seguela, Dirk Inze, Nico Dissmeyer, Yunhai Li, Michael W. Bevan
doi: https://doi.org/10.1101/090563
Hui Dong
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jack Dumenil
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fu-Hao Lu
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Li Na
2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hannes Vanhaeren
3Dept of Plant Systems Biology, VIB, 9052 Gent, Belgium;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christin Naumann
4Leibniz Institute of Plant Biology (IBP), Weinberg 3, D-06120 Halle, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria Klecker
4Leibniz Institute of Plant Biology (IBP), Weinberg 3, D-06120 Halle, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rachel Prior
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caroline Smith
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Neil McKenzie
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gerhard Saalbach
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Liangliang Chen
2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tian Xia
2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nathalie Gonzalez
3Dept of Plant Systems Biology, VIB, 9052 Gent, Belgium;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mathilde Seguela
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
5Institut Jean-Pierre Bourgin, UMR1318, 78000 Versailles, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dirk Inze
3Dept of Plant Systems Biology, VIB, 9052 Gent, Belgium;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nico Dissmeyer
4Leibniz Institute of Plant Biology (IBP), Weinberg 3, D-06120 Halle, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yunhai Li
2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael W. Bevan
1John Innes Centre, Norwich Research Park, Norwich NR4 7QA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

The characteristic shapes and sizes of organs are established by cell proliferation patterns and final cell sizes, but the underlying molecular mechanisms coordinating these are poorly understood. Here we characterize a ubiquitin-activated peptidase called DA1 that limits the duration of cell proliferation during organ growth in Arabidopsis thaliana. The peptidase is activated by two RING E3 ligases, BB and DA2, which are subsequently cleaved by the activated peptidase and destabilized. In the case of BB, cleavage leads to destabilization by the RING E3 ligase PRT1 of the N-end rule pathway. DA1 peptidase activity also cleaves the de-ubiquitylase UBP15, which promotes cell proliferation, and the transcription factors TCP15 and TCP22, which promote cell proliferation proliferation and repress endoreduplication. We propose that DA1 peptidase activity regulates the duration of cell proliferation and the transition to endoreduplication and differentiation during organ formation in plants by coordinating the destabilization of regulatory proteins.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted November 30, 2016.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis
Hui Dong, Jack Dumenil, Fu-Hao Lu, Li Na, Hannes Vanhaeren, Christin Naumann, Maria Klecker, Rachel Prior, Caroline Smith, Neil McKenzie, Gerhard Saalbach, Liangliang Chen, Tian Xia, Nathalie Gonzalez, Mathilde Seguela, Dirk Inze, Nico Dissmeyer, Yunhai Li, Michael W. Bevan
bioRxiv 090563; doi: https://doi.org/10.1101/090563
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis
Hui Dong, Jack Dumenil, Fu-Hao Lu, Li Na, Hannes Vanhaeren, Christin Naumann, Maria Klecker, Rachel Prior, Caroline Smith, Neil McKenzie, Gerhard Saalbach, Liangliang Chen, Tian Xia, Nathalie Gonzalez, Mathilde Seguela, Dirk Inze, Nico Dissmeyer, Yunhai Li, Michael W. Bevan
bioRxiv 090563; doi: https://doi.org/10.1101/090563

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4857)
  • Biochemistry (10802)
  • Bioengineering (8046)
  • Bioinformatics (27314)
  • Biophysics (13987)
  • Cancer Biology (11130)
  • Cell Biology (16062)
  • Clinical Trials (138)
  • Developmental Biology (8788)
  • Ecology (13298)
  • Epidemiology (2067)
  • Evolutionary Biology (17364)
  • Genetics (11689)
  • Genomics (15926)
  • Immunology (11034)
  • Microbiology (26114)
  • Molecular Biology (10655)
  • Neuroscience (56596)
  • Paleontology (418)
  • Pathology (1733)
  • Pharmacology and Toxicology (3005)
  • Physiology (4551)
  • Plant Biology (9644)
  • Scientific Communication and Education (1615)
  • Synthetic Biology (2690)
  • Systems Biology (6979)
  • Zoology (1510)