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The dynamic three-dimensional organization of the diploid yeast genome

Seungsoo Kim, Ivan Liachko, Donna G Brickner, Kate Cook, William S Noble, Jason H Brickner, Jay Shendure, View ORCID ProfileMaitreya J Dunham
doi: https://doi.org/10.1101/091827
Seungsoo Kim
1Department of Genome Sciences, University of Washington, Seattle, WA
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Ivan Liachko
1Department of Genome Sciences, University of Washington, Seattle, WA
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Donna G Brickner
2Department of Molecular Biosciences, Northwestern University, Evanston, IL
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Kate Cook
1Department of Genome Sciences, University of Washington, Seattle, WA
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William S Noble
1Department of Genome Sciences, University of Washington, Seattle, WA
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Jason H Brickner
2Department of Molecular Biosciences, Northwestern University, Evanston, IL
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Jay Shendure
1Department of Genome Sciences, University of Washington, Seattle, WA
3Howard Hughes Medical Institute, University of Washington, Seattle, WA
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Maitreya J Dunham
1Department of Genome Sciences, University of Washington, Seattle, WA
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  • ORCID record for Maitreya J Dunham
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Abstract

The budding yeast Saccharomyces cerevisiae is a long-standing model for the three-dimensional organization of eukaryotic genomes. Even in this well-studied model, it is unclear how homolog pairing in diploids and environment-induced gene relocalization influence overall genome organization. Here, we performed high-throughput chromosome conformation capture on diverged Saccharomyces hybrid diploids to obtain the first global view of chromosome conformation in diploid yeasts. After controlling for the Rabl-like orientation, we observe significant homolog proximity that increased in saturated culture conditions. Surprisingly, we observe a localized increase in homologous interactions between the HAS1 alleles specifically under galactose induction and saturated growth, mediated by association with nuclear pore complexes at the nuclear periphery. Together, these results reveal that the diploid yeast genome has a dynamic and complex 3D organization.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted December 05, 2016.
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The dynamic three-dimensional organization of the diploid yeast genome
Seungsoo Kim, Ivan Liachko, Donna G Brickner, Kate Cook, William S Noble, Jason H Brickner, Jay Shendure, Maitreya J Dunham
bioRxiv 091827; doi: https://doi.org/10.1101/091827
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The dynamic three-dimensional organization of the diploid yeast genome
Seungsoo Kim, Ivan Liachko, Donna G Brickner, Kate Cook, William S Noble, Jason H Brickner, Jay Shendure, Maitreya J Dunham
bioRxiv 091827; doi: https://doi.org/10.1101/091827

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