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Revising mtDNA haplotypes of the ancient Hungarian conquerors with next generation sequencing

Endre Neparáczki, Klaudia Kocsy, Gábor Endre Tóth, Zoltán Maróti, Tibor Kalmár, Péter Bihari, István Nagy, György Pálfi, Erika Molnár, István Raskó, Tibor Török
doi: https://doi.org/10.1101/092239
Endre Neparáczki
1Department of Genetics, University of Szeged, Hungary
5Department of Biological Anthropology University of Szeged, Hungary
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Klaudia Kocsy
1Department of Genetics, University of Szeged, Hungary
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Gábor Endre Tóth
1Department of Genetics, University of Szeged, Hungary
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Zoltán Maróti
2Department of Pediatrics and Pediatric Health Center, University of Szeged, Faculty of Medicine
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Tibor Kalmár
2Department of Pediatrics and Pediatric Health Center, University of Szeged, Faculty of Medicine
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Péter Bihari
3SeqOmics Biotechnology Ltd. Mórahalom, Hungary
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István Nagy
3SeqOmics Biotechnology Ltd. Mórahalom, Hungary
4Institute of Biochemistry, Biological Research Centre, Szeged, Hungary
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György Pálfi
5Department of Biological Anthropology University of Szeged, Hungary
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Erika Molnár
5Department of Biological Anthropology University of Szeged, Hungary
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István Raskó
6Institute of Genetics, Biological Research Centre, Szeged, Hungary
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Tibor Török
1Department of Genetics, University of Szeged, Hungary
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  • For correspondence: torokt@bio.u-szeged.hu
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Abstract

As part of the effort to create a high resolution representative sequence database of the medieval Hungarian conquerors we have resequenced the entire mtDNA genome of 24 published ancient samples with Next Generation Sequencing, whose haplotypes had been previously determined with traditional PCR based methods. We show that PCR based methods are prone to erroneous haplotype or haplogroup determination due to ambiguous sequence reads, and many of the resequenced samples had been classified inaccurately. The SNaPshot method applied with published ancient DNA authenticity criteria is the most straightforward and cheapest PCR based approach for testing a large number of coding region SNP-s, which greatly facilitates correct haplogroup determination.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 07, 2016.
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Revising mtDNA haplotypes of the ancient Hungarian conquerors with next generation sequencing
Endre Neparáczki, Klaudia Kocsy, Gábor Endre Tóth, Zoltán Maróti, Tibor Kalmár, Péter Bihari, István Nagy, György Pálfi, Erika Molnár, István Raskó, Tibor Török
bioRxiv 092239; doi: https://doi.org/10.1101/092239
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Revising mtDNA haplotypes of the ancient Hungarian conquerors with next generation sequencing
Endre Neparáczki, Klaudia Kocsy, Gábor Endre Tóth, Zoltán Maróti, Tibor Kalmár, Péter Bihari, István Nagy, György Pálfi, Erika Molnár, István Raskó, Tibor Török
bioRxiv 092239; doi: https://doi.org/10.1101/092239

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