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Repeated losses of PRDM9-directed recombination despite the conservation of PRDM9 across vertebrates

Zachary Baker, Molly Schumer, Yuki Haba, Lisa Bashkirova, Chris Holland, Gil G. Rosenthal, Molly Przeworski
doi: https://doi.org/10.1101/093096
Zachary Baker
1Dept. of Systems Biology, Columbia University
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  • For correspondence: ztb2002@columbia.edu mp3284@columbia.edu
Molly Schumer
2Dept. of Biological Sciences, Columbia University
3Harvard Society of Fellows, Harvard University
4Centro de Investigaciones Científicas de las Huastecas “Aguazarca”
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Yuki Haba
5Dept. of Evolution, Ecology and Environmental Biology, Columbia University
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Lisa Bashkirova
6Dept. of Biochemistry and Molecular Biophysics, Columbia University
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Chris Holland
4Centro de Investigaciones Científicas de las Huastecas “Aguazarca”
7Dept. of Biology, Texas A&M University
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Gil G. Rosenthal
4Centro de Investigaciones Científicas de las Huastecas “Aguazarca”
7Dept. of Biology, Texas A&M University
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Molly Przeworski
1Dept. of Systems Biology, Columbia University
2Dept. of Biological Sciences, Columbia University
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  • For correspondence: ztb2002@columbia.edu mp3284@columbia.edu
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Abstract

Studies of highly diverged species have revealed two mechanisms by which meiotic recombination is directed to the genome—through PRDM9 binding or by targeting promoter-like features—that lead to dramatically different evolutionary dynamics of hotspots. Here, we identify PRDM9 orthologs from genome and transcriptome data in 225 species. We find the complete PRDM9 ortholog across distantly related vertebrates but, despite this broad conservation, infer a minimum of six partial and three complete losses. Strikingly, taxa carrying the complete ortholog of PRDM9 are precisely those with rapid evolution of its predicted binding affinity, suggesting that all domains are necessary for directing recombination. Indeed, as we show, swordtail fish carrying only a partial but conserved ortholog share recombination properties with PRDM9 knock-outs.

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Posted May 12, 2017.
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Repeated losses of PRDM9-directed recombination despite the conservation of PRDM9 across vertebrates
Zachary Baker, Molly Schumer, Yuki Haba, Lisa Bashkirova, Chris Holland, Gil G. Rosenthal, Molly Przeworski
bioRxiv 093096; doi: https://doi.org/10.1101/093096
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Repeated losses of PRDM9-directed recombination despite the conservation of PRDM9 across vertebrates
Zachary Baker, Molly Schumer, Yuki Haba, Lisa Bashkirova, Chris Holland, Gil G. Rosenthal, Molly Przeworski
bioRxiv 093096; doi: https://doi.org/10.1101/093096

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