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Nodal secures pluripotency upon embryonic stem cell progression from the ground state

Carla Mulas, Tüzer Kalkan, View ORCID ProfileAustin Smith
doi: https://doi.org/10.1101/093880
Carla Mulas
1Wellcome Trust – Medical Research Council Stem Cell Institute, University of Cambridge, Tennis Court Road, CB2 1QR,Cambridge, UK
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Tüzer Kalkan
1Wellcome Trust – Medical Research Council Stem Cell Institute, University of Cambridge, Tennis Court Road, CB2 1QR,Cambridge, UK
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Austin Smith
1Wellcome Trust – Medical Research Council Stem Cell Institute, University of Cambridge, Tennis Court Road, CB2 1QR,Cambridge, UK
2Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1GA, Cambridge, UK
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  • ORCID record for Austin Smith
  • For correspondence: austin.smith@cscr.cam.ac.uk
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SUMMARY

Naïve mouse embryonic stem (ES) cells can readily acquire specific fates, but the cellular and molecular processes that enable lineage specification are poorly characterised. Here we investigated progression from the ES cell ground state in adherent culture. We utilised down-regulation of Rex1::GFPd2 to track loss of ES cell identity. We found that cells that have newly down-regulated this reporter have acquired competence for germline induction. They can also be efficiently specified for different somatic lineages, responding more rapidly than naïve cells to inductive cues. Nodal is a candidate autocrine regulator of pluripotency. Abrogation of Nodal signalling did not substantially alter kinetics of exit from the ES cell state, but accelerated subsequent adoption of neural fate at the expense of other lineages. This effect was evident if Nodal was inhibited prior to extinction of ES cell identity. We suggest that Nodal is pivotal for non-neural competence in cells departing naïve pluripotency.

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Posted December 13, 2016.
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Nodal secures pluripotency upon embryonic stem cell progression from the ground state
Carla Mulas, Tüzer Kalkan, Austin Smith
bioRxiv 093880; doi: https://doi.org/10.1101/093880
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Nodal secures pluripotency upon embryonic stem cell progression from the ground state
Carla Mulas, Tüzer Kalkan, Austin Smith
bioRxiv 093880; doi: https://doi.org/10.1101/093880

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