Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Cell cycle dynamics of chromosomal organisation at single-cell resolution

Takashi Nagano, Yaniv Lubling, Csilla Varnai, Carmel Dudley, Wing Leung, Yael Baran, Netta Mandelson-Cohen, Steven Wingett, View ORCID ProfilePeter Fraser, Amos Tanay
doi: https://doi.org/10.1101/094466
Takashi Nagano
1Nuclear Dynamics Programme, The Babraham Insitute, Cambridge, CB22 3AT,United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yaniv Lubling
2Department of Computer Science and Applied Mathematics, and Department of Biological Regulation, Weizmann Institute, Rehovot, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Csilla Varnai
1Nuclear Dynamics Programme, The Babraham Insitute, Cambridge, CB22 3AT,United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: csilla.varnai@babraham.ac.uk peter.fraser@babraham.ac.uk amos.tanay@weizmann.ac.il
Carmel Dudley
2Department of Computer Science and Applied Mathematics, and Department of Biological Regulation, Weizmann Institute, Rehovot, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wing Leung
1Nuclear Dynamics Programme, The Babraham Insitute, Cambridge, CB22 3AT,United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yael Baran
2Department of Computer Science and Applied Mathematics, and Department of Biological Regulation, Weizmann Institute, Rehovot, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Netta Mandelson-Cohen
2Department of Computer Science and Applied Mathematics, and Department of Biological Regulation, Weizmann Institute, Rehovot, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Steven Wingett
1Nuclear Dynamics Programme, The Babraham Insitute, Cambridge, CB22 3AT,United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter Fraser
1Nuclear Dynamics Programme, The Babraham Insitute, Cambridge, CB22 3AT,United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Peter Fraser
  • For correspondence: csilla.varnai@babraham.ac.uk peter.fraser@babraham.ac.uk amos.tanay@weizmann.ac.il
Amos Tanay
2Department of Computer Science and Applied Mathematics, and Department of Biological Regulation, Weizmann Institute, Rehovot, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: csilla.varnai@babraham.ac.uk peter.fraser@babraham.ac.uk amos.tanay@weizmann.ac.il
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Summary

Chromosomes in proliferating metazoan cells undergo dramatic structural metamorphoses every cell cycle, alternating between a highly condensed mitotic structure facilitating chromosome segregation, and a decondensed interphase structure accommodating transcription, gene silencing and DNA replication. These cyclical structural transformations have been evident under the microscope for over a century, but their molecular-level analysis is still lacking. Here we use single-cell Hi-C to study chromosome conformations in thousands of individual cells, and discover a continuum of cis-interaction profiles that finely position individual cells along the cell cycle. We show that chromosomal compartments, topological domains (TADs), contact insulation and long-range loops, all defined by ensemble Hi-C maps, are governed by distinct cell cycle dynamics. In particular, DNA replication correlates with build-up of compartments and reduction in TAD insulation, while loops are generally stable from G1 through S and G2. Analysing whole genome 3D structural models using haploid cell data, we discover a radial architecture of chromosomal compartments with distinct epigenomic signatures. Our single-cell data creates an essential new paradigm for the re-interpretation of chromosome conformation maps through the prism of the cell cycle.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted December 15, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Cell cycle dynamics of chromosomal organisation at single-cell resolution
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Cell cycle dynamics of chromosomal organisation at single-cell resolution
Takashi Nagano, Yaniv Lubling, Csilla Varnai, Carmel Dudley, Wing Leung, Yael Baran, Netta Mandelson-Cohen, Steven Wingett, Peter Fraser, Amos Tanay
bioRxiv 094466; doi: https://doi.org/10.1101/094466
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Cell cycle dynamics of chromosomal organisation at single-cell resolution
Takashi Nagano, Yaniv Lubling, Csilla Varnai, Carmel Dudley, Wing Leung, Yael Baran, Netta Mandelson-Cohen, Steven Wingett, Peter Fraser, Amos Tanay
bioRxiv 094466; doi: https://doi.org/10.1101/094466

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (2516)
  • Biochemistry (4964)
  • Bioengineering (3466)
  • Bioinformatics (15166)
  • Biophysics (6885)
  • Cancer Biology (5379)
  • Cell Biology (7709)
  • Clinical Trials (138)
  • Developmental Biology (4518)
  • Ecology (7128)
  • Epidemiology (2059)
  • Evolutionary Biology (10206)
  • Genetics (7497)
  • Genomics (9763)
  • Immunology (4821)
  • Microbiology (13174)
  • Molecular Biology (5128)
  • Neuroscience (29354)
  • Paleontology (203)
  • Pathology (835)
  • Pharmacology and Toxicology (1460)
  • Physiology (2127)
  • Plant Biology (4728)
  • Scientific Communication and Education (1008)
  • Synthetic Biology (1337)
  • Systems Biology (4001)
  • Zoology (768)