Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Cohesin dependent compaction of mitotic chromosomes

Stephanie A. Schalbetter, Anton Goloborodko, Geoffrey Fudenberg, Jon M. Belton, Catrina Miles, Miao Yu, Job Dekker, Leonid Mirny, Jon Baxter
doi: https://doi.org/10.1101/094946
Stephanie A. Schalbetter
1Genome Damage and Stability Centre, Science Park Road, University of Sussex, Falmer, Brighton, East Sussex BN1 9RQ, U.K.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anton Goloborodko
3Howard Hughes Medical Institute, Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
4Institute for Medical Engineering and Sciences, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Geoffrey Fudenberg
3Howard Hughes Medical Institute, Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
4Institute for Medical Engineering and Sciences, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jon M. Belton
2Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catrina Miles
1Genome Damage and Stability Centre, Science Park Road, University of Sussex, Falmer, Brighton, East Sussex BN1 9RQ, U.K.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Miao Yu
1Genome Damage and Stability Centre, Science Park Road, University of Sussex, Falmer, Brighton, East Sussex BN1 9RQ, U.K.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Job Dekker
3Howard Hughes Medical Institute, Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leonid Mirny
4Institute for Medical Engineering and Sciences, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jon Baxter
1Genome Damage and Stability Centre, Science Park Road, University of Sussex, Falmer, Brighton, East Sussex BN1 9RQ, U.K.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Structural Maintenance of Chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply-conserved SMC complexes, organize chromosomes in budding yeast. The canonical role of cohesins is to co-align sister chromatids whilst condensins generally compact mitotic chromosomes. We find strikingly different roles in budding yeast mitosis. First, cohesin is responsible for compacting mitotic chromosomes arms, independent of and in addition to its role in sister-chromatid cohesion. Cohesin dependent mitotic chromosome compaction can be fully accounted for through cis-looping of chromatin by loop extrusion. Second, condensin is dispensable for compaction along chromosomal arms and instead plays a specialized role, structuring rDNA and peri-centromeric regions. Our results argue that the conserved mechanism of SMC complexes is to form chromatin loops and that SMC-dependent looping is readily deployed in a range of contexts to functionally organize chromosomes.

  • Cohesin compacts mitotic chromosomes independently of sister chromatid cohesion.

  • Formation of cis-loops by loop extrusion fully accounts for cohesin-mediated compaction.

  • Condensin is not required for mitotic chromosome compaction of yeast chromosome arms

  • Condensin has a focused pre-anaphase role at centromeres and rDNA in yeast

Highlights

Footnotes

  • ↵* Lead experimentalist

  • ↵§ Lead computational data analyst

  • ↵¶ Lead computational modeler

  • Lead contact: Jon Baxter Email: Jon.Baxter{at}sussex.ac.uk Tel: +44 (0)1273 876637

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted December 17, 2016.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Cohesin dependent compaction of mitotic chromosomes
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Cohesin dependent compaction of mitotic chromosomes
Stephanie A. Schalbetter, Anton Goloborodko, Geoffrey Fudenberg, Jon M. Belton, Catrina Miles, Miao Yu, Job Dekker, Leonid Mirny, Jon Baxter
bioRxiv 094946; doi: https://doi.org/10.1101/094946
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Cohesin dependent compaction of mitotic chromosomes
Stephanie A. Schalbetter, Anton Goloborodko, Geoffrey Fudenberg, Jon M. Belton, Catrina Miles, Miao Yu, Job Dekker, Leonid Mirny, Jon Baxter
bioRxiv 094946; doi: https://doi.org/10.1101/094946

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3573)
  • Biochemistry (7517)
  • Bioengineering (5479)
  • Bioinformatics (20675)
  • Biophysics (10257)
  • Cancer Biology (7931)
  • Cell Biology (11578)
  • Clinical Trials (138)
  • Developmental Biology (6563)
  • Ecology (10135)
  • Epidemiology (2065)
  • Evolutionary Biology (13537)
  • Genetics (9497)
  • Genomics (12788)
  • Immunology (7870)
  • Microbiology (19451)
  • Molecular Biology (7613)
  • Neuroscience (41871)
  • Paleontology (306)
  • Pathology (1252)
  • Pharmacology and Toxicology (2179)
  • Physiology (3249)
  • Plant Biology (7005)
  • Scientific Communication and Education (1291)
  • Synthetic Biology (1942)
  • Systems Biology (5405)
  • Zoology (1107)