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A SUMO-Ubiquitin Relay Recruits Proteasomes to Chromosome Axes to Regulate Meiotic Recombination

H.B.D. Prasada Rao, Huanyu Qiao, Shubhang K. Bhatt, Logan R.J. Bailey, Hung D. Tran, Sarah L. Bourne, Wendy Qiu, Anusha Deshpande, Ajay N. Sharma, Connor J. Beebout, Roberto J. Pezza, Neil Hunter
doi: https://doi.org/10.1101/095711
H.B.D. Prasada Rao
1Howard Hughes Medical Institute, University of California, Davis, Davis, California, USA.
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Huanyu Qiao
1Howard Hughes Medical Institute, University of California, Davis, Davis, California, USA.
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Shubhang K. Bhatt
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Logan R.J. Bailey
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Hung D. Tran
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Sarah L. Bourne
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Wendy Qiu
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Anusha Deshpande
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Ajay N. Sharma
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Connor J. Beebout
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
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Roberto J. Pezza
3Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA.
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Neil Hunter
1Howard Hughes Medical Institute, University of California, Davis, Davis, California, USA.
2Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, California, USA.
4Department of Molecular & Cellular Biology, University of California, Davis, Davis, California, USA.
5Department of Cell Biology & Human Anatomy, University of California, Davis, Davis, California, USA.
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  • For correspondence: nhunter@ucdavis.edu
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Abstract

Meiosis produces haploid gametes through a succession of chromosomal events including pairing, synapsis and recombination. Mechanisms that orchestrate these events remain poorly understood. We found that the SUMO-modification and ubiquitin-proteasomes systems regulate the major events of meiotic prophase in mouse. Interdependent localization of SUMO, ubiquitin and proteasomes along chromosome axes was mediated largely by RNF212 and HEI10, two E3 ligases that are also essential for crossover recombination. RNF212-dependent SUMO conjugation effected a checkpoint-like process that stalls recombination by rendering the turnover of a subset of recombination factors dependent on HEI10-mediated ubiquitylation. We propose that SUMO conjugation establishes a precondition for designating crossover sites via selective protein stabilization. Thus, meiotic chromosome axes are hubs for regulated proteolysis via SUMO-dependent control of the ubiquitin-proteasome system.

One Sentence Summary Chromosomal events of meiotic prophase in mouse are regulated by proteasome-dependent protein degradation.

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Posted December 21, 2016.
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A SUMO-Ubiquitin Relay Recruits Proteasomes to Chromosome Axes to Regulate Meiotic Recombination
H.B.D. Prasada Rao, Huanyu Qiao, Shubhang K. Bhatt, Logan R.J. Bailey, Hung D. Tran, Sarah L. Bourne, Wendy Qiu, Anusha Deshpande, Ajay N. Sharma, Connor J. Beebout, Roberto J. Pezza, Neil Hunter
bioRxiv 095711; doi: https://doi.org/10.1101/095711
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A SUMO-Ubiquitin Relay Recruits Proteasomes to Chromosome Axes to Regulate Meiotic Recombination
H.B.D. Prasada Rao, Huanyu Qiao, Shubhang K. Bhatt, Logan R.J. Bailey, Hung D. Tran, Sarah L. Bourne, Wendy Qiu, Anusha Deshpande, Ajay N. Sharma, Connor J. Beebout, Roberto J. Pezza, Neil Hunter
bioRxiv 095711; doi: https://doi.org/10.1101/095711

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