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The interplay of stiffness and force anisotropies drive embryo elongation

View ORCID ProfileThanh TK Vuong-Brender, Martine Ben Amar, Julien Pontabry, Michel Labouesse
doi: https://doi.org/10.1101/095752
Thanh TK Vuong-Brender
1Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biologie du Développement - Institut de Biologie Paris Seine (LBD - IBPS), 75005 Paris, France
2Development and Stem Cells Program, IGBMC, CNRS (UMR7104), INSERM (U964), Université de Strasbourg, 1 rue Laurent Fries, BP10142, 67400 Illkirch, France
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  • ORCID record for Thanh TK Vuong-Brender
Martine Ben Amar
3Laboratoire de Physique Statistique, Ecole Normale Supérieure, UPMC Université Pierre et Marie Curie, Université Paris Diderot, CNRS, 24 rue Lhomond, 75005 Paris, France
4Institut Universitaire de Cancérologie, Faculté de Médecine, Université Pierre et Marie Curie-Paris 6, 91 Bd de l’Hôpital, 75013 Paris, France
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Julien Pontabry
2Development and Stem Cells Program, IGBMC, CNRS (UMR7104), INSERM (U964), Université de Strasbourg, 1 rue Laurent Fries, BP10142, 67400 Illkirch, France
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Michel Labouesse
1Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biologie du Développement - Institut de Biologie Paris Seine (LBD - IBPS), 75005 Paris, France
2Development and Stem Cells Program, IGBMC, CNRS (UMR7104), INSERM (U964), Université de Strasbourg, 1 rue Laurent Fries, BP10142, 67400 Illkirch, France
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Abstract

The morphogenesis of tissues, like the deformation of an object, results from the interplay between their material properties and the mechanical forces exerted on them. Whereas the importance of mechanical forces in influencing cell behaviour is widely recognized, the importance of tissue material properties, in particular stiffness, has received much less attention. Using C. elegans as a model, we examine how both aspects contribute to embryonic elongation. Measuring the opening shape of the epidermal actin cortex after laser nano-ablation, we assess the spatiotemporal changes of actomyosin-dependent force and stiffness along the antero-posterior and dorso-ventral axis. Experimental data and analytical modelling show that myosin II-dependent force anisotropy within the lateral epidermis, and stiffness anisotropy within the fiber-reinforced dorso-ventral epidermis are critical to drive embryonic elongation. Together, our results establish a quantitative link between cortical tension, material properties and morphogenesis of an entire embryo.

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Posted December 20, 2016.
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The interplay of stiffness and force anisotropies drive embryo elongation
Thanh TK Vuong-Brender, Martine Ben Amar, Julien Pontabry, Michel Labouesse
bioRxiv 095752; doi: https://doi.org/10.1101/095752
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The interplay of stiffness and force anisotropies drive embryo elongation
Thanh TK Vuong-Brender, Martine Ben Amar, Julien Pontabry, Michel Labouesse
bioRxiv 095752; doi: https://doi.org/10.1101/095752

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