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Rab5-mediated Yolk Cell Endocytosis modulates Zebrafish Epiboly Biomechanics and Tissue Movements

View ORCID ProfileMaria Marsal, View ORCID ProfileAmayra Hernández-Vega, Philippe-Alexandre Pouille, View ORCID ProfileEnrique Martin-Blanco
doi: https://doi.org/10.1101/097212
Maria Marsal
Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas Parc Cientific de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain
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Amayra Hernández-Vega
Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas Parc Cientific de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain
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Philippe-Alexandre Pouille
Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas Parc Cientific de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain
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Enrique Martin-Blanco
Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas Parc Cientific de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain
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  • For correspondence: embbmc@ibmb.csic.es
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Summary

Morphogenetic processes demand the coordinated allocation of cells and tissues to their final destination in a spatio-temporal controlled way. Identifying how these morphogenetic movements are directed and implemented is essential for understanding morphogenesis. Topographical and scalar differences in adhesion and contractility within and between cells are essential, yet, the role that membrane remodeling may play remains less clear. To clarify how surface turnover and dynamics may modulate tissue arrangements we studied epiboly in the zebrafish. During epiboly the blastoderm expands as a result of an asymmetry of mechanical tension along the embryo surface. In this scenario, we found that the membrane removal by macropinocytosis of the external yolk cell syncytial layer (E-YSL) ahead of the blastoderm is key for epiboly progression In early zebrafish embryos, the activity of the GTPase Rab5ab was essential for endocytosis, and interference in its expression exclusively in the yolk cell resulted in the reduction of yolk cell actomyosin contractility, the disruption of cortical and internal yolk flows, a disequilibrium in force balance and as a result epiboly impairment. We conclude that regulated membrane remodeling is crucial for directing cell and tissue mechanics and coordinating morphogenetic movements during epiboly.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted June 09, 2017.
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Rab5-mediated Yolk Cell Endocytosis modulates Zebrafish Epiboly Biomechanics and Tissue Movements
Maria Marsal, Amayra Hernández-Vega, Philippe-Alexandre Pouille, Enrique Martin-Blanco
bioRxiv 097212; doi: https://doi.org/10.1101/097212
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Rab5-mediated Yolk Cell Endocytosis modulates Zebrafish Epiboly Biomechanics and Tissue Movements
Maria Marsal, Amayra Hernández-Vega, Philippe-Alexandre Pouille, Enrique Martin-Blanco
bioRxiv 097212; doi: https://doi.org/10.1101/097212

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