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grID: A CRISPR-Cas9 guide RNA Database and Resource for Genome-Editing

View ORCID ProfileVinod Jaskula-Ranga, View ORCID ProfileDonald J. Zack
doi: https://doi.org/10.1101/097352
Vinod Jaskula-Ranga
1Department of Ophthalmology, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, USA
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  • For correspondence: vinny1@jhmi.edu dzack@jhmi.edu
Donald J. Zack
1Department of Ophthalmology, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, USA
2Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, USA
3Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, USA
4Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, USA
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ABSTRACT

CRISPR-Cas9 genome-editing is a revolutionary technology that is transforming biological research. The explosive growth and advances in CRISPR research over the last few years, coupled with the potential for clinical applications and therapeutics, is heralding a new era for genome engineering. To further support this technology platform and to provide a universal CRISPR annotation system, we introduce the grID database (http://crispr.technology), an extensive compilation of gRNA properties including sequence and variations, thermodynamic parameters, off-target analyses, and alternative PAM sites, among others. To aid in the design of optimal gRNAs, the website is integrated with other prominent databases, providing a wealth of additional resources to guide users from in silico analysis through experimental CRISPR targeting. Here, we make available all the tools, protocols, and plasmids that are needed for successful CRISPR-based genome targeting.

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Posted December 30, 2016.
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grID: A CRISPR-Cas9 guide RNA Database and Resource for Genome-Editing
Vinod Jaskula-Ranga, Donald J. Zack
bioRxiv 097352; doi: https://doi.org/10.1101/097352
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grID: A CRISPR-Cas9 guide RNA Database and Resource for Genome-Editing
Vinod Jaskula-Ranga, Donald J. Zack
bioRxiv 097352; doi: https://doi.org/10.1101/097352

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