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D-Alanine esterification of teichoic acids contributes to Lactobacillus plantarum mediated intestinal peptidase expression and Drosophila growth promotion upon chronic undernutrition

Renata C. Matos, Hugo Gervais, Pauline Joncour, Martin Schwarzer, Benjamin Gillet, Maria Elena Martino, Pascal Courtin, Sandrine Hughes, Marie-Pierre Chapot-Chartier, View ORCID ProfileFrançois Leulier
doi: https://doi.org/10.1101/098434
Renata C. Matos
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, 69364 Lyon Cedex 07, France
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Hugo Gervais
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, 69364 Lyon Cedex 07, France
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Pauline Joncour
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, 69364 Lyon Cedex 07, France
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Martin Schwarzer
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, 69364 Lyon Cedex 07, France
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Benjamin Gillet
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, 69364 Lyon Cedex 07, France
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Maria Elena Martino
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, 69364 Lyon Cedex 07, France
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Pascal Courtin
2Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France
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Sandrine Hughes
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, 69364 Lyon Cedex 07, France
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Marie-Pierre Chapot-Chartier
2Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France
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François Leulier
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, 69364 Lyon Cedex 07, France
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  • ORCID record for François Leulier
  • For correspondence: francois.leulier@ens-lyon.fr
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Summary

The microbial environment influence animal physiology. However, the underlying molecular mechanisms of such functional interactions are largely undefined. Previously, we showed that upon chronic undernutrition, strains of Lactobacillus plantarum, a dominant commensal partner of Drosophila, promote host juvenile growth and maturation partly via enhanced expression of intestinal peptidases. By screening a transposon insertion library of Lactobacillus plantarum in gnotobiotic Drosophila larvae, we identify a bacterial cell wall modifying machinery encoded by the pbpX2-dltXABCD operon that is critical to enhance host digestive capabilities and promote growth and maturation. Deletion of this operon leads to bacterial cell wall alteration with a complete loss of teichoic acids D-alanylation. We thus conclude that teichoic acids modifications participate in commensal-host interactions and specifically, D-alanine esterification of teichoic acids contributes to optimal L. plantarum mediated intestinal peptidase expression and Drosophila juvenile growth upon chronic undernutrition.

Highlights

  • - LpNC8 mutant library screening identifies genes affecting Drosophila growth promotion.

  • - pbpX2-dlt operon is required for D-alanylation of teichoic acids and Drosophila growth.

  • - Deleting the pbpX2-dlt operon alters host intestinal peptidase expression.

  • - Peptidoglycan and pbpX2-dlt dependent signals are required for LpNC8 mediated growth promotion.

eTOC blurb Animals establish interactions with their microbial communities that shape many aspects of their physiology including juvenile growth. However, the underlying molecular mechanisms are largely undefined. Matos et al. reveal that bacterial teichoic acids modifications contribute to host juvenile growth promotion.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 05, 2017.
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D-Alanine esterification of teichoic acids contributes to Lactobacillus plantarum mediated intestinal peptidase expression and Drosophila growth promotion upon chronic undernutrition
Renata C. Matos, Hugo Gervais, Pauline Joncour, Martin Schwarzer, Benjamin Gillet, Maria Elena Martino, Pascal Courtin, Sandrine Hughes, Marie-Pierre Chapot-Chartier, François Leulier
bioRxiv 098434; doi: https://doi.org/10.1101/098434
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D-Alanine esterification of teichoic acids contributes to Lactobacillus plantarum mediated intestinal peptidase expression and Drosophila growth promotion upon chronic undernutrition
Renata C. Matos, Hugo Gervais, Pauline Joncour, Martin Schwarzer, Benjamin Gillet, Maria Elena Martino, Pascal Courtin, Sandrine Hughes, Marie-Pierre Chapot-Chartier, François Leulier
bioRxiv 098434; doi: https://doi.org/10.1101/098434

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