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The 2D-structure of the T. brucei pre-edited RPS12 mRNA is not affected by macromolecular crowding

W.-Matthias Leeder, Stephan Voskuhl, View ORCID ProfileH. Ulrich Göringer
doi: https://doi.org/10.1101/099200
W.-Matthias Leeder
1Molecular Genetics; Darmstadt University of Technology; Schnittspahnstraße 10; 64287 Darmstadt; Germany
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Stephan Voskuhl
1Molecular Genetics; Darmstadt University of Technology; Schnittspahnstraße 10; 64287 Darmstadt; Germany
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H. Ulrich Göringer
1Molecular Genetics; Darmstadt University of Technology; Schnittspahnstraße 10; 64287 Darmstadt; Germany
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  • ORCID record for H. Ulrich Göringer
  • For correspondence: goringer@bio.tu-darmstadt.de
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Abstract

Mitochondrial transcript maturation in African trypanosomes requires RNA editing to convert nucleotide-deficient pre-mRNAs into translatable mRNAs. The different pre-mRNAs have been shown to adopt highly stable 2D-folds, however, it is not known whether these structures resemble the in vivo folds given the extreme “crowding” conditions within the mitochondrion. Here we analyze the effects of macromolecular crowding on the structure of the mitochondrial RPS12 pre-mRNA. We use polyethylene glycol as a macromolecular cosolute and monitor the structure of the RNA globally and with nucleotide resolution. We demonstrate that crowding has no impact on the 2D-fold and we conclude that the MFE-structure in dilute solvent conditions represents a good proxy for the folding of the pre-mRNA in its mitochondrial solvent context.

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Posted January 12, 2017.
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The 2D-structure of the T. brucei pre-edited RPS12 mRNA is not affected by macromolecular crowding
W.-Matthias Leeder, Stephan Voskuhl, H. Ulrich Göringer
bioRxiv 099200; doi: https://doi.org/10.1101/099200
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The 2D-structure of the T. brucei pre-edited RPS12 mRNA is not affected by macromolecular crowding
W.-Matthias Leeder, Stephan Voskuhl, H. Ulrich Göringer
bioRxiv 099200; doi: https://doi.org/10.1101/099200

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