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Polycomb-Mediated Chromatin Loops Revealed by a Sub-Kilobase Resolution Chromatin Interaction Map

View ORCID ProfileKyle P. Eagen, Erez Lieberman Aiden, Roger D. Kornberg
doi: https://doi.org/10.1101/099804
Kyle P. Eagen
aDepartment of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
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  • ORCID record for Kyle P. Eagen
  • For correspondence: eagen@northwestern.edu kornberg@stanford.edu
Erez Lieberman Aiden
bThe Center for Genome Architecture, Baylor College of Medicine, Houston, TX 77030, USA
cDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
dCenter for Theoretical Biological Physics, Rice University, Houston, TX 77005, USA
eDepartment of Computer Science, Rice University, Houston, TX 77005, USA
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Roger D. Kornberg
aDepartment of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
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  • For correspondence: eagen@northwestern.edu kornberg@stanford.edu
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ABSTRACT

The locations of chromatin loops in Drosophila were determined by Hi-C (chemical cross-linking, restriction digestion, ligation, and high-throughput DNA sequencing). Whereas most loop boundaries or “anchors” are associated with CTCF protein in mammals, loop anchors in Drosophila were found most often in association with the polycomb group (PcG) protein Polycomb (Pc), a subunit of Polycomb Repressive Complex 1 (PRC1). Loops were frequently located within domains of PcG-repressed chromatin. Promoters located at PRC1 loop anchors regulate some of the most important developmental genes and are less likely to be expressed than those not at PRC1 loop anchors. Although DNA looping has most commonly been associated with enhancer-promoter communication, our results indicate that loops are also associated with gene repression.

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Posted January 12, 2017.
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Polycomb-Mediated Chromatin Loops Revealed by a Sub-Kilobase Resolution Chromatin Interaction Map
Kyle P. Eagen, Erez Lieberman Aiden, Roger D. Kornberg
bioRxiv 099804; doi: https://doi.org/10.1101/099804
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Polycomb-Mediated Chromatin Loops Revealed by a Sub-Kilobase Resolution Chromatin Interaction Map
Kyle P. Eagen, Erez Lieberman Aiden, Roger D. Kornberg
bioRxiv 099804; doi: https://doi.org/10.1101/099804

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