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Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells

Isidro Abreu, Ángela Saéz, Rosario Castro-Rodríguez, Viviana Escudero, Benjamín Rodríguez-Haas, Marta Senovilla, Camille Larue, Daniel Grolimund, Manuel Tejada-Jiménez, Juan Imperial, Manuel González-Guerrero
doi: https://doi.org/10.1101/102426
Isidro Abreu
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Ángela Saéz
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Rosario Castro-Rodríguez
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Viviana Escudero
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Benjamín Rodríguez-Haas
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Marta Senovilla
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Camille Larue
2 ECOLAB. Université de Toulouse. CNRS, INPT, UPS. France
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Daniel Grolimund
3 Paul Scherrer Institute. Swiss Light Source. microXAS Beamline Project. CH-5232 Villigen. Switzerland.
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Manuel Tejada-Jiménez
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Juan Imperial
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
4Consejo Superior de Investigaciones Científicas. Madrid. Spain.
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Manuel González-Guerrero
1 Centro de Biotecnología y GenóSmica de Plantas (UPM-INIA). Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Abstract:

Zinc is a micronutrient required for symbiotic nitrogen fixation. It has been proposed that in model legume Medicago truncatula, zinc is delivered in a similar fashion as iron, i.e. by the root vasculature into the nodule and released in the infection/differentiation zone. There, zinc transporters must introduce this element into rhizobia-infected cells to metallate the apoproteins that use zinc as a cofactor. MtZIP6 (Medtr4g083570) is a M. truncatula Zinc-Iron Permease (ZIP) that is expressed only in roots and nodules, with the highest expression levels in the infection/differentiation zone. Immunolocalization studies indicate that it is located in the plasma membrane of rhizobia-infected cells in the nodule. Down-regulating MtZIP6 expression levels with RNAi does not result in any strong phenotype when plants are being watered with mineral nitrogen. However, these silenced plants displayed severe growth defects when they depended on nitrogen fixed by their nodules, as a consequence of the loss of 80% of their nitrogenase activity. The reduction of this activity was not the result of iron not reaching the nodule, but an indirect effect of zinc being retained in the infection/differentiation zone and not reaching the cytosol of rhizobia-infected cells. These data are consistent with a model in which MtZIP6 would be responsible for zinc uptake by rhizobia-infected nodule cells in the infection/differentiation zone.

Footnotes

  • Present address Benjamín Rodríguez-Haas current address: Red de Estudios Moleculares Avanzados, Cluster Biomimic®. Instituto de Ecología A. C. Carretera Antigua a Coatepec, 351. El Haya, 91070 Xalapa, Veracruz, Mexico. Manuel Tejada-Jiménez current address: Department of Biochemistry and Molecular Biology. Universidad de Córdoba. Campus de Rabanales. Edificio Severo Ochoa. Córdoba. Spain.

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Posted January 24, 2017.
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Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells
Isidro Abreu, Ángela Saéz, Rosario Castro-Rodríguez, Viviana Escudero, Benjamín Rodríguez-Haas, Marta Senovilla, Camille Larue, Daniel Grolimund, Manuel Tejada-Jiménez, Juan Imperial, Manuel González-Guerrero
bioRxiv 102426; doi: https://doi.org/10.1101/102426
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Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells
Isidro Abreu, Ángela Saéz, Rosario Castro-Rodríguez, Viviana Escudero, Benjamín Rodríguez-Haas, Marta Senovilla, Camille Larue, Daniel Grolimund, Manuel Tejada-Jiménez, Juan Imperial, Manuel González-Guerrero
bioRxiv 102426; doi: https://doi.org/10.1101/102426

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