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Medicago truncatula MOT1.3 is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules

Manuel Tejada-Jiménez, Patricia Gil-Díez, Javier León-Mediavilla, Jiangqi Wen, Kirankumar S. Mysore, Juan Imperial, Manuel González-Guerrero
doi: https://doi.org/10.1101/102517
Manuel Tejada-Jiménez
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA). Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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  • For correspondence: q62tejim@uco.es manuel.gonzalez@upm.es
Patricia Gil-Díez
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA). Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Javier León-Mediavilla
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA). Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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Jiangqi Wen
2Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401.
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Kirankumar S. Mysore
2Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401.
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Juan Imperial
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA). Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
3Consejo Superior de Investigaciones Científicas. Madrid. Spain.
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Manuel González-Guerrero
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA). Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid). Spain.
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  • For correspondence: q62tejim@uco.es manuel.gonzalez@upm.es
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Summary

  • Molybdenum, as a component of the iron-molybdenum cofactor of nitrogenase, is essential for symbiotic nitrogen fixation. This nutrient has to be provided by the host plant through molybdate transporters.

  • Members of the molybdate transporters family MOT1 were identified in the model legume Medicago truncatula and their expression in nodules determined. Yeast toxicity assays, confocal microscopy, and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the one M. truncatula MOT1 family member expressed specifically in nodules.

  • Among the five MOT1 members present in M. truncatula genome, MtMOT1.3 is the only one uniquely expressed in nodules. MtMOT1.3 shows molybdate transport capabilities when expressed in yeast. Immunolocalization studies revealed that MtMOT1.3 is located in the plasma membrane of nodule cells. A mot1.3-1 knockout mutant showed an impaired growth concomitant with a reduction in nitrogenase activity. This phenotype was rescued by increasing molybdate concentrations in the nutritive solution, or upon addition of an assimilable nitrogen source. Furthermore, mot1.3-1 plants transformed with a functional copy of MtMOT1.3 showed a wild type-like phenotype.

  • These data are consistent with a model in which MtMOT1.3 would be responsible for introducing molybdate into nodule cells, which will be later used to synthesize functional nitrogenase.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 31, 2017.
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Medicago truncatula MOT1.3 is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules
Manuel Tejada-Jiménez, Patricia Gil-Díez, Javier León-Mediavilla, Jiangqi Wen, Kirankumar S. Mysore, Juan Imperial, Manuel González-Guerrero
bioRxiv 102517; doi: https://doi.org/10.1101/102517
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Medicago truncatula MOT1.3 is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules
Manuel Tejada-Jiménez, Patricia Gil-Díez, Javier León-Mediavilla, Jiangqi Wen, Kirankumar S. Mysore, Juan Imperial, Manuel González-Guerrero
bioRxiv 102517; doi: https://doi.org/10.1101/102517

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