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A Molecular Signature for Anastasis, Recovery from the Brink of Apoptotic Cell Death

Gongping Sun, Elmer Guzman, Hongjun Robin Zhou, Kenneth S. Kosik, View ORCID ProfileDenise J. Montell
doi: https://doi.org/10.1101/102640
Gongping Sun
Molecular, Cellular, and Developmental Biology Department Neuroscience Research Institute University of California Santa Barbara, CA 93106
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Elmer Guzman
Molecular, Cellular, and Developmental Biology Department Neuroscience Research Institute University of California Santa Barbara, CA 93106
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Hongjun Robin Zhou
Molecular, Cellular, and Developmental Biology Department Neuroscience Research Institute University of California Santa Barbara, CA 93106
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Kenneth S. Kosik
Molecular, Cellular, and Developmental Biology Department Neuroscience Research Institute University of California Santa Barbara, CA 93106
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Denise J. Montell
Molecular, Cellular, and Developmental Biology Department Neuroscience Research Institute University of California Santa Barbara, CA 93106
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  • ORCID record for Denise J. Montell
  • For correspondence: denise.montell@lifesci.ucsb.edu
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Abstract

During apoptosis, executioner caspase activity has been considered a point of no return. However, recent studies show that cells can survive caspase activation following transient apoptotic stimuli, a process named anastasis. To identify a molecular signature, we performed whole transcriptome RNA sequencing of untreated, apoptotic, and recovering HeLa cells. We found that anastasis is an active, two-stage program. During the early stage, cells transition from growth-arrested to growing. In the late stage, cells change from proliferating to migratory. Strikingly, some early recovery mRNAs were elevated first during apoptosis, implying that dying cells poise to recover, even while still under apoptotic stress. Furthermore, TGFβ-induced Snail expression is required for anastasis, and recovering cells exhibit prolonged elevation of pro-angiogenic factors. This study demonstrates similarities in the anastasis genes, pathways, and cell behaviors to those activated in wound healing. This study identifies a repertoire of potential targets for therapeutic manipulation of this process.

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Posted January 24, 2017.
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A Molecular Signature for Anastasis, Recovery from the Brink of Apoptotic Cell Death
Gongping Sun, Elmer Guzman, Hongjun Robin Zhou, Kenneth S. Kosik, Denise J. Montell
bioRxiv 102640; doi: https://doi.org/10.1101/102640
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A Molecular Signature for Anastasis, Recovery from the Brink of Apoptotic Cell Death
Gongping Sun, Elmer Guzman, Hongjun Robin Zhou, Kenneth S. Kosik, Denise J. Montell
bioRxiv 102640; doi: https://doi.org/10.1101/102640

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