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Medicago truncatula copper transporter 1 (MtCOPT1) delivers copper for symbiotic nitrogen fixation

Marta Senovilla, Rosario Castro-Rodríguez, Isidro Abreu, Viviana Escudero, Igor Kryvoruchko, Michael K. Udvardi, Juan Imperial, Manuel González Guerrero
doi: https://doi.org/10.1101/104554
Marta Senovilla
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA).Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid).Spain.
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Rosario Castro-Rodríguez
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA).Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid).Spain.
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Isidro Abreu
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA).Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid).Spain.
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Viviana Escudero
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA).Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid).Spain.
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Igor Kryvoruchko
2Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
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  • For correspondence: manuel.gonzalez@upm.es
Michael K. Udvardi
2Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
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Juan Imperial
2Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
3Consejo Superior de Investigaciones Científicas. Madrid.Spain.
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Manuel González Guerrero
1Centro de Biotecnología y Genómica de Plantas (UPM-INIA).Campus de Montegancedo. Universidad Politécnica de Madrid. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid).Spain.
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  • For correspondence: manuel.gonzalez@upm.es
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Summary

• Copper is an essential nutrient for symbiotic nitrogen fixation. This element is delivered by the host plant to the nodule, where membrane copper transporter would introduce it into the cell to synthesize cupro-proteins.

• COPT family members in model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy, and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M.truncatula COPT family member.

• Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early fixation zones. Loss of MtCOPT1 function results in a copper-mitigated reduction of biomass production when the plant obtains its nitrogen exclusively from symbiotic nitrogen fixation. Mutation of MtCOPT1 results in diminished nitrogenase activity in nodules, likely an indirect effect from the loss of a copper-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids.

• These data are consistent with a model in which MtCOPT1 transports copper from the apoplast into nodule cells to provide copper for essential metabolic processes associated with symbiotic nitrogen fixation.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 31, 2017.
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Medicago truncatula copper transporter 1 (MtCOPT1) delivers copper for symbiotic nitrogen fixation
Marta Senovilla, Rosario Castro-Rodríguez, Isidro Abreu, Viviana Escudero, Igor Kryvoruchko, Michael K. Udvardi, Juan Imperial, Manuel González Guerrero
bioRxiv 104554; doi: https://doi.org/10.1101/104554
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Medicago truncatula copper transporter 1 (MtCOPT1) delivers copper for symbiotic nitrogen fixation
Marta Senovilla, Rosario Castro-Rodríguez, Isidro Abreu, Viviana Escudero, Igor Kryvoruchko, Michael K. Udvardi, Juan Imperial, Manuel González Guerrero
bioRxiv 104554; doi: https://doi.org/10.1101/104554

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