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Daisyfield gene drive systems harness repeated genomic elements as a generational clock to limit spread

John Min, View ORCID ProfileCharleston Noble, View ORCID ProfileDevora Najjar, View ORCID ProfileKevin M. Esvelt
doi: https://doi.org/10.1101/104877
John Min
1MIT Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
2Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA
3Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
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Charleston Noble
3Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
4Program for Evolutionary Dynamics, Harvard University, Cambridge, Massachusetts, USA
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Devora Najjar
1MIT Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
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Kevin M. Esvelt
1MIT Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
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Abstract

Methods of altering wild populations are most useful when inherently limited to local geographic areas. Here we describe a novel form of gene drive based on the introduction of multiple copies of an engineered ‘daisy’ sequence into repeated elements of the genome. Each introduced copy encodes guide RNAs that target one or more engineered loci carrying the CRISPR nuclease gene and the desired traits. When organisms encoding a drive system are released into the environment, each generation of mating with wild-type organisms will reduce the average number of the guide RNA elements per ‘daisyfield’ organism by half, serving as a generational clock. The loci encoding the nuclease and payload will exhibit drive only as long as a single copy remains, placing an inherent limit on the extent of spread.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted February 06, 2017.
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Daisyfield gene drive systems harness repeated genomic elements as a generational clock to limit spread
John Min, Charleston Noble, Devora Najjar, Kevin M. Esvelt
bioRxiv 104877; doi: https://doi.org/10.1101/104877
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Daisyfield gene drive systems harness repeated genomic elements as a generational clock to limit spread
John Min, Charleston Noble, Devora Najjar, Kevin M. Esvelt
bioRxiv 104877; doi: https://doi.org/10.1101/104877

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