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PGC-1α coordinates with Bcl-2 to control cell cycle in U251 cells through reducing ROS

Kun Yao, Xufeng Fu, Du Xing, Yan Li, Bing Han, Chen Zexi, Shanshan Yang, Ran Wei, Jiaqi Zhou, Qinghua Cui
doi: https://doi.org/10.1101/111567
Kun Yao
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
2Key Laboratory for tumor molecular biology in Yunnan Province, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
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Xufeng Fu
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
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Du Xing
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
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Yan Li
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
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Bing Han
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
3Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, P.R. China;
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Chen Zexi
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
3Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, P.R. China;
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Shanshan Yang
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
2Key Laboratory for tumor molecular biology in Yunnan Province, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
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Ran Wei
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
2Key Laboratory for tumor molecular biology in Yunnan Province, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
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Jiaqi Zhou
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
2Key Laboratory for tumor molecular biology in Yunnan Province, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
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Qinghua Cui
1School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
2Key Laboratory for tumor molecular biology in Yunnan Province, Yunnan University, Kunming, Yunnan, 650091, P.R. China;
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  • For correspondence: cuiqinghua@ynu.edu.cn
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Abstract

B-cell lymphoma 2 (Bcl-2) has a dual function, acting both as an oncogene and an anti-tumor gene. It is well known that Bcl-2 exerts its tumor promoting function through the mitochondrial pathway. However, the mechanism by which Bcl-2 suppresses tumor formation is not well understood. We have previously shown that Bcl-2 inhibits cell cycle progression from the G0/G1 to the S phase after serum starvation, and that quiescent Bcl-2 expressing cells maintained a significant lower level of mitochondrial reactive oxygen species (ROS) than the control cells. Based on the fact that ROS mediate cell cycle progression, and are controlled by peroxisome proliferator-activated receptor-γ co-activator 1α (PGC-1α), a key molecule induced by prolonged starvation and involved in mitochondrial metabolism, we hypothesized that PGC-1α might be related with the cell cycle function of Bcl-2. Here, we showed that PGC-1α was upregulated upon Bcl-2 overexpression and downregulated following Bcl-2 knockdown during serum starvation. Knockdown of PGC-1α activated Bcl-2 expression. Taken together, our results suggest that after serum depletion, PGC-la might coordinate with Bcl-2 to reduce ROS, which in turn delay cell cycle progression.

Summary statement PGC-1α coordinate with Bcl-2 delay cell cycle progression to reduce ROS after serum depletion in human glioma U251 cells.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted February 24, 2017.
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PGC-1α coordinates with Bcl-2 to control cell cycle in U251 cells through reducing ROS
Kun Yao, Xufeng Fu, Du Xing, Yan Li, Bing Han, Chen Zexi, Shanshan Yang, Ran Wei, Jiaqi Zhou, Qinghua Cui
bioRxiv 111567; doi: https://doi.org/10.1101/111567
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PGC-1α coordinates with Bcl-2 to control cell cycle in U251 cells through reducing ROS
Kun Yao, Xufeng Fu, Du Xing, Yan Li, Bing Han, Chen Zexi, Shanshan Yang, Ran Wei, Jiaqi Zhou, Qinghua Cui
bioRxiv 111567; doi: https://doi.org/10.1101/111567

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