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A microfluidic biodisplay

Francesca Volpetti, Ekaterina Petrova, Sebastian J. Maerkl
doi: https://doi.org/10.1101/112110
Francesca Volpetti
Institute of Bioengineering, School of Engineering, EcolePolytechniqueFederalede Lausanne
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Ekaterina Petrova
Institute of Bioengineering, School of Engineering, EcolePolytechniqueFederalede Lausanne
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Sebastian J. Maerkl
Institute of Bioengineering, School of Engineering, EcolePolytechniqueFederalede Lausanne
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  • For correspondence: sebastian.maerkl@epfl.ch
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ABSTRACT

Synthetically engineered cells are powerful and potentially useful biosensors, but it remains difficult to deploy such systems due to practical difficulties and biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves as an interface between an engineered cellular system, environment, and user. We created a biodisplay consisting of 768 individually programmable biopixels and demonstrated that it can perform multiplexed, continuous environmental monitoring. The biodisplay detected 10 μg/l sodium-arsenite in tap water using a research grade fluorescent microscope, and reported arsenic contamination down to 20 μg/l with an easy to interpret “skull and crossbones” symbol detectable with a low-cost USB microscope or by eye. No chemical or biological material is released by the biodisplay preventing environmental contamination. The microfluidic biodisplay thus provides a practical solution for the deployment and application of engineered cellular systems.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted February 27, 2017.
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A microfluidic biodisplay
Francesca Volpetti, Ekaterina Petrova, Sebastian J. Maerkl
bioRxiv 112110; doi: https://doi.org/10.1101/112110
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A microfluidic biodisplay
Francesca Volpetti, Ekaterina Petrova, Sebastian J. Maerkl
bioRxiv 112110; doi: https://doi.org/10.1101/112110

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