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Large-scale simultaneous measurement of epitopes and transcriptomes in single cells

View ORCID ProfileMarlon Stoeckius, View ORCID ProfileChristoph Hafemeister, William Stephenson, Brian Houck-Loomis, Pratip K. Chattopadhyay, Harold Swerdlow, Rahul Satija, View ORCID ProfilePeter Smibert
doi: https://doi.org/10.1101/113068
Marlon Stoeckius
1New York Genome Center, New York, NY
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  • For correspondence: mstoeckius@nygenome.org
Christoph Hafemeister
1New York Genome Center, New York, NY
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William Stephenson
1New York Genome Center, New York, NY
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Brian Houck-Loomis
1New York Genome Center, New York, NY
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Pratip K. Chattopadhyay
3New York University Medical Center, New York, NY
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Harold Swerdlow
1New York Genome Center, New York, NY
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Rahul Satija
1New York Genome Center, New York, NY
2New York University Center for Genomics and Systems Biology, New York, NY
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Peter Smibert
1New York Genome Center, New York, NY
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Abstract

Recent high-throughput single-cell sequencing approaches have been transformative for understanding complex cell populations, but are unable to provide additional phenotypic information, such as protein levels of cell-surface markers. Using oligonucleotide-labeled antibodies, we integrate measurements of cellular proteins and transcriptomes into an efficient, sequencing-based readout of single cells. This method is compatible with existing single-cell sequencing approaches and will readily scale as the throughput of these methods increase.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 07, 2017.
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Large-scale simultaneous measurement of epitopes and transcriptomes in single cells
Marlon Stoeckius, Christoph Hafemeister, William Stephenson, Brian Houck-Loomis, Pratip K. Chattopadhyay, Harold Swerdlow, Rahul Satija, Peter Smibert
bioRxiv 113068; doi: https://doi.org/10.1101/113068
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Large-scale simultaneous measurement of epitopes and transcriptomes in single cells
Marlon Stoeckius, Christoph Hafemeister, William Stephenson, Brian Houck-Loomis, Pratip K. Chattopadhyay, Harold Swerdlow, Rahul Satija, Peter Smibert
bioRxiv 113068; doi: https://doi.org/10.1101/113068

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