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Genotyping of zebrafish embryos fixed by paraformaldehyde

Xiaojie Yang, View ORCID ProfileQilin Gu
doi: https://doi.org/10.1101/118331
Xiaojie Yang
1Department of Cardiovascular Sciences, Houston Methodist Research Institute, Houston, Texas 77030, USA
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Qilin Gu
1Department of Cardiovascular Sciences, Houston Methodist Research Institute, Houston, Texas 77030, USA
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Summary

The rapid generation of various species and strains of zebrafish using CRISPR/Cas9 technology has dramatically accelerated the interrogation of gene function in vivo. So far, several approaches for genotyping of unfixed genome-modified animals have been successfully developed, such as Surveyor assays, T7 endonuclease 1 (T7E1) assays, High Resolution Melt Analysis (HRMA) and PAGE-based genotyping approach. However, there is few published genotyping protocol for the embryos of lethal zebrafish mutants fixed by paraformaldehyde. We have designed this genotyping protocol so that it can be performed in a single step with reliable results. The protocol covers all steps to obtain the genotypes.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 19, 2017.
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Genotyping of zebrafish embryos fixed by paraformaldehyde
Xiaojie Yang, Qilin Gu
bioRxiv 118331; doi: https://doi.org/10.1101/118331
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Genotyping of zebrafish embryos fixed by paraformaldehyde
Xiaojie Yang, Qilin Gu
bioRxiv 118331; doi: https://doi.org/10.1101/118331

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