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Semisynthetic pH-sensitive fluorophores for imaging exocytosis and endocytosis

Magalie Martineau, Agila Somasundaram, Jonathan B. Grimm, Todd D. Gruber, Daniel Choquet, Justin W. Taraska, Luke D. Lavis, David Perrais
doi: https://doi.org/10.1101/118505
Magalie Martineau
1University of Bordeaux, F-33000 Bordeaux, France
2Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France
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Agila Somasundaram
4National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA.
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Jonathan B. Grimm
5Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, USA.
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Todd D. Gruber
5Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, USA.
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Daniel Choquet
1University of Bordeaux, F-33000 Bordeaux, France
2Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France
3Bordeaux Imaging Center, UMS 3420 CNRS, Université de Bordeaux, US 4 INSERM, F-33000, Bordeaux, France
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Justin W. Taraska
4National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA.
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Luke D. Lavis
5Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, USA.
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David Perrais
1University of Bordeaux, F-33000 Bordeaux, France
2Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France
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  • For correspondence: david.perrais@u-bordeaux lavisl@janelia.hhmi.org justin.taraska@nih.gov
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Abstract

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe ‘semisynthetic’ pH-sensitive protein conjugates that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.

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Posted March 25, 2017.
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Semisynthetic pH-sensitive fluorophores for imaging exocytosis and endocytosis
Magalie Martineau, Agila Somasundaram, Jonathan B. Grimm, Todd D. Gruber, Daniel Choquet, Justin W. Taraska, Luke D. Lavis, David Perrais
bioRxiv 118505; doi: https://doi.org/10.1101/118505
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Semisynthetic pH-sensitive fluorophores for imaging exocytosis and endocytosis
Magalie Martineau, Agila Somasundaram, Jonathan B. Grimm, Todd D. Gruber, Daniel Choquet, Justin W. Taraska, Luke D. Lavis, David Perrais
bioRxiv 118505; doi: https://doi.org/10.1101/118505

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