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H+- and Na+-elicited swift changes of the microtubule system in the biflagellated green alga Chlamydomonas

Yi Liu, Mike Visetsouk, Michelle Mynlieff, Hongmin Qin, View ORCID ProfileKarl F. Lechtreck, View ORCID ProfilePinfen Yang
doi: https://doi.org/10.1101/121137
Yi Liu
1Department of Biological Sciences, Marquette University, 530 N. 15th St. Milwaukee WI, USA
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Mike Visetsouk
1Department of Biological Sciences, Marquette University, 530 N. 15th St. Milwaukee WI, USA
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Michelle Mynlieff
1Department of Biological Sciences, Marquette University, 530 N. 15th St. Milwaukee WI, USA
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Hongmin Qin
2Department of Biology, Texas A & M University, 525 Lubbock St. College Station, TX 77843-16 3258, USA
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Karl F. Lechtreck
3Department of Cellular Biology, University of Georgia, 1000 Cedar St. Athens, GA, USA
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Pinfen Yang
1Department of Biological Sciences, Marquette University, 530 N. 15th St. Milwaukee WI, USA
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  • ORCID record for Pinfen Yang
  • For correspondence: Pinfen.yang@marquette.edu
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Abstract

The microtubule cytoskeletal system is integral to diverse cellular processes. Although microtubules are known for dynamic instability, the system is tightly controlled in typical interphase animal cells. In contrast, diverse evidence suggests that the system is mercurial in the unicellular fresh water green alga, Chlamydomonas, but intense autofluorescence from photosynthesis pigments has hindered the investigation. By expressing a bright fluorescent reporter protein at the endogenous level, we demonstrate in real time discreet sweeping changes in algal microtubules elicited by fluctuation of intracellular H+ and Na+. These results suggest disparate sensitivity of this vital yet delicate system in diverse organisms; and illuminate how pH may drive crucial cellular processes; how plants respond to, and perhaps sense stresses; and how many species could be susceptible to accelerated changes in global environments.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 27, 2017.
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H+- and Na+-elicited swift changes of the microtubule system in the biflagellated green alga Chlamydomonas
Yi Liu, Mike Visetsouk, Michelle Mynlieff, Hongmin Qin, Karl F. Lechtreck, Pinfen Yang
bioRxiv 121137; doi: https://doi.org/10.1101/121137
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H+- and Na+-elicited swift changes of the microtubule system in the biflagellated green alga Chlamydomonas
Yi Liu, Mike Visetsouk, Michelle Mynlieff, Hongmin Qin, Karl F. Lechtreck, Pinfen Yang
bioRxiv 121137; doi: https://doi.org/10.1101/121137

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