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Cross-talk between active DNA demethylation, resetting of cellular metabolism and shoot apical growth in poplar bud break

Daniel Conde, Mariano Perales, Anne-Laure Le Gac, Christopher Dervinis, Matias Kirst, Stéphane Maury, Pablo González-Melendi, Isabel Allona
doi: https://doi.org/10.1101/122119
Daniel Conde
aCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcón, 28223 Madrid, Spain
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Mariano Perales
aCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcón, 28223 Madrid, Spain
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Anne-Laure Le Gac
bLBLGC EA1207, USC 1328 INRA, University Orléans, 45067 Orléans, France
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Christopher Dervinis
cSchool of Forest Resources and Conservation, University of Florida, Gainesville, Florida 32611, USA
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Matias Kirst
cSchool of Forest Resources and Conservation, University of Florida, Gainesville, Florida 32611, USA
dUniversity of Florida Genetics Institute, University of Florida, Gainesville, FL 32611, USA
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Stéphane Maury
bLBLGC EA1207, USC 1328 INRA, University Orléans, 45067 Orléans, France
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Pablo González-Melendi
aCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcón, 28223 Madrid, Spain
eDepartamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM), 28040 Madrid, Spain.
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Isabel Allona
aCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcón, 28223 Madrid, Spain
eDepartamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM), 28040 Madrid, Spain.
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  • For correspondence: isabel.allona@upm.es
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Abstract

Annual dormancy-growth cycle is a developmental and physiological process essential for the survival of temperate and boreal forests. Seasonal control of shoot growth in woody perennials requires specific genetic programs integrated with the environmental signals. The environmental-controlled mechanisms that regulate the shift between winter dormancy to growth promoting genetic program are still unknown. Here, we show that dynamics in genomic DNA methylation (gDNA) levels regulate dormancy-growth cycle in poplar. We proved that the reactivation of cell division in the apical shoot that lead bud break process in spring, is preceded by a progressive reduction of gDNA methylation in apex tissue. We also identified that the induction in apex tissue of a chilling-dependent poplar DEMETER-LIKE 10 (PtaDML10) DNA demethylase precedes shoot growth reactivation. Transgenic poplars showing down-regulation of PtaDML8/10 caused delayed bud break. Genome wide transcriptome and methylome analysis and data mining revealed the gene targets of active DML-dependent DNA demethylation genetically associated to bud break. These data point to a chilling dependent-DEMETER-like DNA demethylase controlling the genetic shift from winter dormancy to a condition that promotes shoot apical vegetative growth in poplar.

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Posted April 03, 2017.
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Cross-talk between active DNA demethylation, resetting of cellular metabolism and shoot apical growth in poplar bud break
Daniel Conde, Mariano Perales, Anne-Laure Le Gac, Christopher Dervinis, Matias Kirst, Stéphane Maury, Pablo González-Melendi, Isabel Allona
bioRxiv 122119; doi: https://doi.org/10.1101/122119
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Cross-talk between active DNA demethylation, resetting of cellular metabolism and shoot apical growth in poplar bud break
Daniel Conde, Mariano Perales, Anne-Laure Le Gac, Christopher Dervinis, Matias Kirst, Stéphane Maury, Pablo González-Melendi, Isabel Allona
bioRxiv 122119; doi: https://doi.org/10.1101/122119

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