Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

Katarzyna Jezierska-Wozniak, Seweryn Lipiński, Łukasz Grabarczyk, Monika Barczewska, Aleksandra Habich, Joanna Wojtkiewicz, Wojciech Maksymowicz
doi: https://doi.org/10.1101/122382
Katarzyna Jezierska-Wozniak
1Department of Neurology and Neurosurgery, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
2Laboratory for Regenerative Medicine, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Seweryn Lipiński
3Department of Electric and Power Engineering, Electronics and Automatics; Faculty of Technical Sciences; University of Warmia and Mazury in Olsztyn
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Łukasz Grabarczyk
1Department of Neurology and Neurosurgery, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Monika Barczewska
1Department of Neurology and Neurosurgery, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aleksandra Habich
1Department of Neurology and Neurosurgery, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
2Laboratory for Regenerative Medicine, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joanna Wojtkiewicz
2Laboratory for Regenerative Medicine, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
4Department of Pathophysiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Poland
5Foundation for the Nerve Cells Regeneration.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wojciech Maksymowicz
1Department of Neurology and Neurosurgery, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The objective of our study was to develop novel techniques for investigations of cell motility, and to assess whether the electric field of the therapeutic spinal cord stimulation system used in vivo contributes to the migration of human mesenchymal stem cells (hMSCs) in vitro.

We have investigated electrotaxis of bone marrow-derived MSCs using pulsed electric field (PEF) in range 16-80 mV/mm and frequency 130 Hz and 240 Hz. The PEF-related dynamics of the cell surface glycosylation was evaluated using six plant lectins.

PEF at physiological levels (10mV/mm; 130 Hz) did not influence cellular motility in vitro, what may correspond to the maintenance of the transplanted cells at the lesion site in vivo. Increase of the PEF intensity and frequency above physiological levels resulted in the increase in the cellular migration rate in vitro. PEF elevated above physiological intensity and frequency (40-80 mV/mm; 240 Hz), but not at physiological levels, resulted in changes of the cell surface glycosylation.

We find the described approach as convenient for investigations and for the in vitro modeling of the cellular systems intended for the regenerative cell transplantations in vivo. Probing cell surface glycomes may provide valuable biomarkers to assess competence of transplanted cells.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted March 30, 2017.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation
Katarzyna Jezierska-Wozniak, Seweryn Lipiński, Łukasz Grabarczyk, Monika Barczewska, Aleksandra Habich, Joanna Wojtkiewicz, Wojciech Maksymowicz
bioRxiv 122382; doi: https://doi.org/10.1101/122382
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation
Katarzyna Jezierska-Wozniak, Seweryn Lipiński, Łukasz Grabarczyk, Monika Barczewska, Aleksandra Habich, Joanna Wojtkiewicz, Wojciech Maksymowicz
bioRxiv 122382; doi: https://doi.org/10.1101/122382

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2434)
  • Biochemistry (4796)
  • Bioengineering (3335)
  • Bioinformatics (14704)
  • Biophysics (6649)
  • Cancer Biology (5180)
  • Cell Biology (7440)
  • Clinical Trials (138)
  • Developmental Biology (4374)
  • Ecology (6890)
  • Epidemiology (2057)
  • Evolutionary Biology (9930)
  • Genetics (7351)
  • Genomics (9542)
  • Immunology (4570)
  • Microbiology (12702)
  • Molecular Biology (4954)
  • Neuroscience (28382)
  • Paleontology (199)
  • Pathology (809)
  • Pharmacology and Toxicology (1394)
  • Physiology (2025)
  • Plant Biology (4516)
  • Scientific Communication and Education (978)
  • Synthetic Biology (1302)
  • Systems Biology (3919)
  • Zoology (729)