Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Extensive alternative splicing transitions during postnatal skeletal muscle development are required for calcium handling functions

Amy E. Brinegar, Zheng Xia, James A. Loehr, Wei Li, George G. Rodney, View ORCID ProfileThomas A. Cooper
doi: https://doi.org/10.1101/124230
Amy E. Brinegar
1Department of Molecular & Cellular Biology, Baylor College of Medicine, Houston, TX 77030
4Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zheng Xia
1Department of Molecular & Cellular Biology, Baylor College of Medicine, Houston, TX 77030
2Division of Biostatistics, Dan L Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
James A. Loehr
3Department of Molecular Physiology & Biophysics, Baylor College of Medicine, Houston, TX 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wei Li
1Department of Molecular & Cellular Biology, Baylor College of Medicine, Houston, TX 77030
2Division of Biostatistics, Dan L Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
George G. Rodney
3Department of Molecular Physiology & Biophysics, Baylor College of Medicine, Houston, TX 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas A. Cooper
1Department of Molecular & Cellular Biology, Baylor College of Medicine, Houston, TX 77030
3Department of Molecular Physiology & Biophysics, Baylor College of Medicine, Houston, TX 77030
4Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Thomas A. Cooper
  • For correspondence: tcooper@bcm.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Postnatal development of skeletal muscle is a highly dynamic period of tissue remodeling. Here we used RNA-seq to identify transcriptome changes from late embryonic to adult mouse muscle and demonstrate that alternative splicing developmental transitions impact muscle physiology. The first two weeks after birth are particularly dynamic for differential gene expression and AS transitions, and calciumhandling functions are significantly enriched among genes that undergo alternative splicing. We focused on the postnatal splicing transitions of three calcineurin A genes, calcium-dependent phosphatases that regulate multiple aspects of muscle biology. Redirected splicing of calcineurin A to the fetal isoforms in adult muscle and in differentiated C2C12 slows the timing of muscle relaxation, promotes nuclear localization of calcineurin targets Nfatc3 and Nfatc2, and affects expression of Nfatc transcription targets. The results demonstrate a previously unknown specificity of calcineurin isoforms as well as the broader impact of AS during muscle postnatal development.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted April 05, 2017.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Extensive alternative splicing transitions during postnatal skeletal muscle development are required for calcium handling functions
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Extensive alternative splicing transitions during postnatal skeletal muscle development are required for calcium handling functions
Amy E. Brinegar, Zheng Xia, James A. Loehr, Wei Li, George G. Rodney, Thomas A. Cooper
bioRxiv 124230; doi: https://doi.org/10.1101/124230
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Extensive alternative splicing transitions during postnatal skeletal muscle development are required for calcium handling functions
Amy E. Brinegar, Zheng Xia, James A. Loehr, Wei Li, George G. Rodney, Thomas A. Cooper
bioRxiv 124230; doi: https://doi.org/10.1101/124230

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4672)
  • Biochemistry (10336)
  • Bioengineering (7655)
  • Bioinformatics (26284)
  • Biophysics (13497)
  • Cancer Biology (10664)
  • Cell Biology (15408)
  • Clinical Trials (138)
  • Developmental Biology (8485)
  • Ecology (12802)
  • Epidemiology (2067)
  • Evolutionary Biology (16819)
  • Genetics (11380)
  • Genomics (15458)
  • Immunology (10593)
  • Microbiology (25164)
  • Molecular Biology (10197)
  • Neuroscience (54382)
  • Paleontology (399)
  • Pathology (1665)
  • Pharmacology and Toxicology (2889)
  • Physiology (4332)
  • Plant Biology (9223)
  • Scientific Communication and Education (1585)
  • Synthetic Biology (2554)
  • Systems Biology (6769)
  • Zoology (1459)