Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Formin-Dependent Adhesions are Required for Invasion by Epithelial Tissues

View ORCID ProfileTim B. Fessenden, Yvonne Beckham, Mathew Perez-Neut, Aparajita H. Chourasia, Kay F. Macleod, Patrick W. Oakes, Margaret L. Gardel
doi: https://doi.org/10.1101/124297
Tim B. Fessenden
1Institute for Biophysical Dynamics, James Franck Institute, and Department of Physics, University of Chicago, Chicago, IL 60637
2Committee on Cancer Biology, University of Chicago, Chicago, IL 60637
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tim B. Fessenden
Yvonne Beckham
1Institute for Biophysical Dynamics, James Franck Institute, and Department of Physics, University of Chicago, Chicago, IL 60637
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mathew Perez-Neut
2Committee on Cancer Biology, University of Chicago, Chicago, IL 60637
3Ben May Department of Cancer Research, University of Chicago, Chicago, IL 60637
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aparajita H. Chourasia
2Committee on Cancer Biology, University of Chicago, Chicago, IL 60637
3Ben May Department of Cancer Research, University of Chicago, Chicago, IL 60637
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kay F. Macleod
3Ben May Department of Cancer Research, University of Chicago, Chicago, IL 60637
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patrick W. Oakes
1Institute for Biophysical Dynamics, James Franck Institute, and Department of Physics, University of Chicago, Chicago, IL 60637
4Current Address: Department of Physics and Astronomy, Department of Biology, University of Rochester, Rochester, NY 14627
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Margaret L. Gardel
1Institute for Biophysical Dynamics, James Franck Institute, and Department of Physics, University of Chicago, Chicago, IL 60637
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Developing tissues change shape, and tumors initiate spreading, through collective cell motility. Conserved mechanisms by which tissues initiate motility into their surroundings are not known. We investigated cytoskeletal regulators during collective invasion by mouse tumor organoids and epithelial MDCK acini undergoing branching morphogenesis. Inhibition of formins, but not Arp2/3, prevented the formation of migrating cell fronts in both cell types. MDCK cells depleted of the formin protein Dia1 formed polarized acini and could execute planar cell motility, either within the acinus or in 2D scattering assays. However, Dia1 was required to form protrusions into the collagen matrix. Live imaging of actin, myosin, and collagen in control acini revealed adhesions that deformed individual collagen fibrils, while Dia1-depleted acini exhibited unstable adhesions with minimal collagen deformation. This work identifies Dia1-mediated adhesions as essential regulators of tissue shape changes, through their role in focal adhesion maturation.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted April 06, 2017.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Formin-Dependent Adhesions are Required for Invasion by Epithelial Tissues
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Formin-Dependent Adhesions are Required for Invasion by Epithelial Tissues
Tim B. Fessenden, Yvonne Beckham, Mathew Perez-Neut, Aparajita H. Chourasia, Kay F. Macleod, Patrick W. Oakes, Margaret L. Gardel
bioRxiv 124297; doi: https://doi.org/10.1101/124297
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Formin-Dependent Adhesions are Required for Invasion by Epithelial Tissues
Tim B. Fessenden, Yvonne Beckham, Mathew Perez-Neut, Aparajita H. Chourasia, Kay F. Macleod, Patrick W. Oakes, Margaret L. Gardel
bioRxiv 124297; doi: https://doi.org/10.1101/124297

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4235)
  • Biochemistry (9136)
  • Bioengineering (6784)
  • Bioinformatics (24001)
  • Biophysics (12129)
  • Cancer Biology (9534)
  • Cell Biology (13778)
  • Clinical Trials (138)
  • Developmental Biology (7636)
  • Ecology (11702)
  • Epidemiology (2066)
  • Evolutionary Biology (15513)
  • Genetics (10644)
  • Genomics (14326)
  • Immunology (9483)
  • Microbiology (22839)
  • Molecular Biology (9090)
  • Neuroscience (48995)
  • Paleontology (355)
  • Pathology (1482)
  • Pharmacology and Toxicology (2570)
  • Physiology (3846)
  • Plant Biology (8331)
  • Scientific Communication and Education (1471)
  • Synthetic Biology (2296)
  • Systems Biology (6192)
  • Zoology (1301)