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Vitamin and amino acid auxotrophy in anaerobic consortia operating under methanogenic condition

Valerie Hubalek, Moritz Buck, BoonFei Tan, Julia Foght, Annelie Wendeberg, David Berry, Stefan Bertilsson, Alexander Eiler
doi: https://doi.org/10.1101/128660
Valerie Hubalek
1Department of Ecology and Genetics, Limnology and Science for Life Laboratory, Uppsala University, Sweden
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Moritz Buck
1Department of Ecology and Genetics, Limnology and Science for Life Laboratory, Uppsala University, Sweden
2NBIS, National Bioinformatics Infrastructure Sweden, Uppsala, Sweden
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BoonFei Tan
3Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
4Center for Environmental Sensing and Modeling, Singapore-MIT Alliance for Research and Technology
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Julia Foght
3Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
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Annelie Wendeberg
5Centre for Environmental Research, Environmental Microbiology, Leipzig, Germany
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David Berry
6Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of Vienna, Vienna, Austria
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Stefan Bertilsson
1Department of Ecology and Genetics, Limnology and Science for Life Laboratory, Uppsala University, Sweden
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Alexander Eiler
1Department of Ecology and Genetics, Limnology and Science for Life Laboratory, Uppsala University, Sweden
7eDNA solutions AB, Mölndal, Sweden
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  • For correspondence: alexander.eiler@icloud.com
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Abstract

Syntrophy among Archaea and Bacteria facilitates the anaerobic degradation of organic compounds to CH4 and CO2. Particularly during aliphatic and aromatic hydrocarbon mineralization, as in crude oil reservoirs and petroleum-contaminated sediments, metabolic interactions between obligate mutualistic microbial partners are of central importance1. Using micro-manipulation combined with shotgun metagenomic approaches, we disentangled the genomes of complex consortia inside a short chain alkane-degrading cultures operating under methanogenic conditions. Metabolic reconstruction revealed that only a small fraction of genes in the metagenome-assembled genomes of this study, encode the capacity for fermentation of alkanes facilitated by energy conservation linked to H2 metabolism. Instead, inferred lifestyles based on scavenging anabolic products and intermediate fermentation products derived from detrital biomass was a common feature in the consortia. Additionally, inferred auxotrophy for vitamins and amino acids suggests that the hydrocarbon-degrading microbial assemblages are structured and maintained by multiple interactions beyond the canonical H2-producing and syntrophic alkane degrader–methanogen partnership2. Our study uncovers the complexity of ‘interactomes’ within microbial consortia mediating hydrocarbon transformation under anaerobic conditions.

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Posted April 19, 2017.
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Vitamin and amino acid auxotrophy in anaerobic consortia operating under methanogenic condition
Valerie Hubalek, Moritz Buck, BoonFei Tan, Julia Foght, Annelie Wendeberg, David Berry, Stefan Bertilsson, Alexander Eiler
bioRxiv 128660; doi: https://doi.org/10.1101/128660
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Vitamin and amino acid auxotrophy in anaerobic consortia operating under methanogenic condition
Valerie Hubalek, Moritz Buck, BoonFei Tan, Julia Foght, Annelie Wendeberg, David Berry, Stefan Bertilsson, Alexander Eiler
bioRxiv 128660; doi: https://doi.org/10.1101/128660

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