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A dry method for preserving tear protein samples

Weiwei Qin, Chan Zhao, Linpei Zhang, Ting Wang, Youhe Gao
doi: https://doi.org/10.1101/131060
Weiwei Qin
1Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing, China
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Chan Zhao
2Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China
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Linpei Zhang
1Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing, China
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Ting Wang
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Youhe Gao
1Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing, China
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  • For correspondence: gaoyouhe@bnu.edu.cn
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Abstract

Tears covering the ocular surface is an important bio-fluid containing thousands of molecules, including proteins, lipids, metabolites, nucleic acids, and electrolytes. Tears are valuable resources for biomarker research of ocular and even systemic diseases. For application in biomarker studies, tear samples should ideally be stored using a simple, low-cost, and efficient method along with the patient’s medical records. For this purpose, we developed a novel Schirmer’s strip-based dry method that allows for storage of tear samples in vacuum bags at room temperature. Using this method, tear protein patterns can also be preserved. Liquid chromatography-mass spectrometry/mass spectrometry analysis of proteins recovered from the dry method and traditional wet method showed no significant difference. Some tissue/organ enriched proteins were identified in tear, thus tear might be a good window for monitoring the change of these tissues or organs. This dry method facilitates sample transportation and enables the storage of tear samples on a large scale, increasing the availability of samples for studying disease biomarkers in tears.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 26, 2017.
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A dry method for preserving tear protein samples
Weiwei Qin, Chan Zhao, Linpei Zhang, Ting Wang, Youhe Gao
bioRxiv 131060; doi: https://doi.org/10.1101/131060
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A dry method for preserving tear protein samples
Weiwei Qin, Chan Zhao, Linpei Zhang, Ting Wang, Youhe Gao
bioRxiv 131060; doi: https://doi.org/10.1101/131060

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