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PHRED-1 is a divergent neurexin-1 homolog that organizes muscle fibers and patterns organs during regeneration

Carolyn E. Adler, Alejandro Sánchez Alvarado
doi: https://doi.org/10.1101/131789
Carolyn E. Adler
aStowers Institute for Medical Research and Howard Hughes Medical Institute 1000 E. 50th Street Kansas City, MO 64110 USA,
bDepartment of Molecular Medicine Cornell University 930 Campus Road, VMC C3-167 Ithaca, NY 14853 USA,
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  • For correspondence: asa@stowers.org cea88@cornell.edu
Alejandro Sánchez Alvarado
aStowers Institute for Medical Research and Howard Hughes Medical Institute 1000 E. 50th Street Kansas City, MO 64110 USA,
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  • For correspondence: asa@stowers.org
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Abstract

Regeneration of body parts requires the replacement of multiple cell types. To dissect this complex process, we utilized planarian flatworms that are capable of regenerating any tissue after amputation. An RNAi screen for genes involved in regeneration of the pharynx identified a novel gene, Pharynx regeneration defective-1 (PHRED-1) as essential for normal pharynx regeneration. PHRED-1 is a predicted transmembrane protein containing EGF, Laminin G, and WD40 domains, is expressed in muscle, and has predicted homologs restricted to other lophotrochozoan species. Knockdown of PHRED-1 causes abnormal regeneration of muscle fibers in both the pharynx and body wall muscle. In addition to defects in muscle regeneration, knockdown of PHRED-1 or the bHLH transcription factor MyoD also causes defects in muscle and intestinal regeneration. Together, our data demonstrate that muscle plays a key role in restoring the structural integrity of closely associated organs, and in planarians it may form a scaffold that facilitates normal intestinal branching.

Highlights -PHRED-1 is a predicted transmembrane protein that contains Laminin G, EGF and WD40 domains

-PHRED-1 is required for normal muscle patterning during regeneration

-phred-1 is expressed in muscle cells

-Muscle forms an essential scaffold for regeneration

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted April 28, 2017.
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PHRED-1 is a divergent neurexin-1 homolog that organizes muscle fibers and patterns organs during regeneration
Carolyn E. Adler, Alejandro Sánchez Alvarado
bioRxiv 131789; doi: https://doi.org/10.1101/131789
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PHRED-1 is a divergent neurexin-1 homolog that organizes muscle fibers and patterns organs during regeneration
Carolyn E. Adler, Alejandro Sánchez Alvarado
bioRxiv 131789; doi: https://doi.org/10.1101/131789

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