Abstract
We have developed a oblique version of the light sheet microscope to do volumetric imaging of the whole brains at high spatial resolution. Tissue clearing using aqueous techniques reduces the structural rigidity of the brain, to overcome this, we developed a gelatin based re-embedding procedure that makes the brain rigid enough to be sectioned. A few examples of whole brain volumetric images are shown using the oblique light sheet tomography.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted May 05, 2017.
