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Aubergine and piRNAs repress the proto-oncogene Cbl for germline stem cell self-renewal

Patricia Rojas-Ríos, Aymeric Chartier, Martine Simonelig
doi: https://doi.org/10.1101/134304
Patricia Rojas-Ríos
1mRNA Regulation and Development, Institute of Human Genetics, UMR9002 CNRS-Université de Montpellier, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
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Aymeric Chartier
1mRNA Regulation and Development, Institute of Human Genetics, UMR9002 CNRS-Université de Montpellier, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
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Martine Simonelig
1mRNA Regulation and Development, Institute of Human Genetics, UMR9002 CNRS-Université de Montpellier, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
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  • For correspondence: Martine.Simonelig@igh.cnrs.fr
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Abstract

PIWI proteins have essential roles in germ cells and stem cell lineages. In Drosophila, Piwi is required in somatic niche cells and germline stem cells (GSCs) for GSC self-renewal and differentiation. Whether and how other PIWI proteins are involved in GSC biology remains unknown. Here, we show that Aubergine (Aub), another PIWI protein, is intrinsically required in GSCs for their self-renewal and differentiation. Aub loading with piRNAs is essential for these functions. The major role of Aub is in self-renewal and depends on mRNA regulation. We identify the Cbl proto-oncogene, a regulator of mammalian hematopoietic stem cells, as a novel GSC differentiation factor. Aub represses Cbl mRNA translation for GSC self-renewal, and does so through recruitment of the CCR4-NOT complex. This study reveals the role of piRNAs and PIWI proteins in translational repression for stem cell homeostasis and highlights piRNAs as major post-transcriptional regulators in key developmental decisions.

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Posted May 04, 2017.
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Aubergine and piRNAs repress the proto-oncogene Cbl for germline stem cell self-renewal
Patricia Rojas-Ríos, Aymeric Chartier, Martine Simonelig
bioRxiv 134304; doi: https://doi.org/10.1101/134304
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Aubergine and piRNAs repress the proto-oncogene Cbl for germline stem cell self-renewal
Patricia Rojas-Ríos, Aymeric Chartier, Martine Simonelig
bioRxiv 134304; doi: https://doi.org/10.1101/134304

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