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FCS in closed systems and application for membrane nanotubes

Yanfei Jiang, Artem Melnykov, Elliot E. Elson
doi: https://doi.org/10.1101/134742
Yanfei Jiang
1Department of Biochemistry and Molecular Biophysics, School of Medicine, Washington University in St. Louis
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Artem Melnykov
1Department of Biochemistry and Molecular Biophysics, School of Medicine, Washington University in St. Louis
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Elliot E. Elson
1Department of Biochemistry and Molecular Biophysics, School of Medicine, Washington University in St. Louis
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Abstract

In the present study, we developed the fluorescence correlation spectroscopy theory for closed systems with either periodic or reflective boundaries. The illumination could be any arbitrary function. We tested our theory with simulated data of both boundary conditions. We also tested the theory with experimental data of membrane nanotubes, whose circular direction is a closed system. The result shows that the correlation function for nanotubes falls between 1D and 2D diffusion model. The fitting with our model gives an accurate recovery of the diffusion time and nanotube radius. We also give some examples of single molecule experiments for which our theory can be potentially useful.

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Posted May 05, 2017.
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FCS in closed systems and application for membrane nanotubes
Yanfei Jiang, Artem Melnykov, Elliot E. Elson
bioRxiv 134742; doi: https://doi.org/10.1101/134742
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FCS in closed systems and application for membrane nanotubes
Yanfei Jiang, Artem Melnykov, Elliot E. Elson
bioRxiv 134742; doi: https://doi.org/10.1101/134742

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