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Boosting the localization precision of dSTORM by biocompatible metal-dielectric coated glass coverslips

View ORCID ProfileHannah S. Heil, Benjamin Schreiber, Monika Emmerling, Sven Hoefling, Martin Kamp, Markus Sauer, Katrin G. Heinze
doi: https://doi.org/10.1101/136739
Hannah S. Heil
1Rudolf Virchow Center, Research Center for Experimental Biomedicine, University of Würzburg, Josef-Schneider-Str.2, 97080 Würzburg, Germany
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  • ORCID record for Hannah S. Heil
Benjamin Schreiber
1Rudolf Virchow Center, Research Center for Experimental Biomedicine, University of Würzburg, Josef-Schneider-Str.2, 97080 Würzburg, Germany
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Monika Emmerling
2Technische Physik, Physikalisches Institut and Wilhelm Conrad Röntgen-Center for Complex Material Systems, University of Würzburg, Am Hubland, 97074 Würzburg, Germany
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Sven Hoefling
2Technische Physik, Physikalisches Institut and Wilhelm Conrad Röntgen-Center for Complex Material Systems, University of Würzburg, Am Hubland, 97074 Würzburg, Germany
3SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, United Kingdom
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Martin Kamp
2Technische Physik, Physikalisches Institut and Wilhelm Conrad Röntgen-Center for Complex Material Systems, University of Würzburg, Am Hubland, 97074 Würzburg, Germany
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Markus Sauer
4Department of Biotechnology and Biophysics, Biozentrum, University of Würzburg, Am Hubland, 97074 Würzburg, Germany
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Katrin G. Heinze
1Rudolf Virchow Center, Research Center for Experimental Biomedicine, University of Würzburg, Josef-Schneider-Str.2, 97080 Würzburg, Germany
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Abstract

Super-resolution techniques such as direct Stochastic Optical Reconstruction Microscopy (dSTORM) have become versatile and well-established tools for biological imaging over the last century. Here, we theoretically and experimentally show that clever combination of different fluorescence modalities allows further improvements. We found that the interaction of fluorophores with plasmonic surfaces boost super-resolution performance in dSTORM approaches as it allows for tailoring the excitation and emission properties. The strength of the approach is that no further specialized microscope setup is required as the described enhancement solely rely on metal-dielectric coated glass coverslips that are straightforward to fabricate. Such biocompatible plasmonic nanolayers enhance the signal-to-noise ratio of dSTORM, and thus sharpens the localization precision by a factor of two.

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Posted May 11, 2017.
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Boosting the localization precision of dSTORM by biocompatible metal-dielectric coated glass coverslips
Hannah S. Heil, Benjamin Schreiber, Monika Emmerling, Sven Hoefling, Martin Kamp, Markus Sauer, Katrin G. Heinze
bioRxiv 136739; doi: https://doi.org/10.1101/136739
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Boosting the localization precision of dSTORM by biocompatible metal-dielectric coated glass coverslips
Hannah S. Heil, Benjamin Schreiber, Monika Emmerling, Sven Hoefling, Martin Kamp, Markus Sauer, Katrin G. Heinze
bioRxiv 136739; doi: https://doi.org/10.1101/136739

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