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Natural chromatin is heterogeneous and self associates in vitro

View ORCID ProfileShujun Cai, Yajiao Song, Chen Chen, View ORCID ProfileJian Shi, View ORCID ProfileLu Gan
doi: https://doi.org/10.1101/139543
Shujun Cai
1Department of Biological Sciences and Centre for BioImaging Sciences, National University of Singapore, Singapore 117543
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Yajiao Song
1Department of Biological Sciences and Centre for BioImaging Sciences, National University of Singapore, Singapore 117543
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Chen Chen
1Department of Biological Sciences and Centre for BioImaging Sciences, National University of Singapore, Singapore 117543
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Jian Shi
1Department of Biological Sciences and Centre for BioImaging Sciences, National University of Singapore, Singapore 117543
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Lu Gan
1Department of Biological Sciences and Centre for BioImaging Sciences, National University of Singapore, Singapore 117543
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  • For correspondence: lu@anaphase.org
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ABSTRACT

The 30-nm fiber is commonly found in oligonucleosome arrays in vitro but rarely found in chromatin within nuclei. To determine how chromatin high-order structure is controlled, we used cryo-ET to study the undigested natural chromatin released from cells that do not have evidence of 30-nm fibers in vivo: picoplankton and yeast. In the presence of divalent cations, most of the chromatin from both organisms is compacted into a large mass. Rare irregular 30-nm fibers do form at the periphery of this mass, some of which include face-to-face interactions. In the absence of divalent cations, picoplankton chromatin decondenses into open zigzags. By contrast, yeast chromatin mostly remains compact with looser nucleosome packing, even after treatment with histone-deacetylase inhibitor. The 3-D configuration of natural chromatin is therefore sensitive to the local environment, but generally nonpermissive of regular motifs, even at the level of oligonucleosomes.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted May 18, 2017.
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Natural chromatin is heterogeneous and self associates in vitro
Shujun Cai, Yajiao Song, Chen Chen, Jian Shi, Lu Gan
bioRxiv 139543; doi: https://doi.org/10.1101/139543
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Natural chromatin is heterogeneous and self associates in vitro
Shujun Cai, Yajiao Song, Chen Chen, Jian Shi, Lu Gan
bioRxiv 139543; doi: https://doi.org/10.1101/139543

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