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High-Definition Electronic Genome Maps from Single Molecule Data

John S. Oliver, Anthony Catalano, Jennifer R. Davis, Boris S. Grinberg, Timothy E. Hutchins, Michael D. Kaiser, Steve Nurnberg, Jay M. Sage, Leah Seward, Gregory Simelgor, Nathan K. Weiner, Barrett Bready
doi: https://doi.org/10.1101/139840
John S. Oliver
Nabsys 2.0, LLC, Providence, RI 02903
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Anthony Catalano
Nabsys 2.0, LLC, Providence, RI 02903
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Jennifer R. Davis
Nabsys 2.0, LLC, Providence, RI 02903
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Boris S. Grinberg
Nabsys 2.0, LLC, Providence, RI 02903
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Timothy E. Hutchins
Nabsys 2.0, LLC, Providence, RI 02903
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Michael D. Kaiser
Nabsys 2.0, LLC, Providence, RI 02903
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Steve Nurnberg
Nabsys 2.0, LLC, Providence, RI 02903
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Jay M. Sage
Nabsys 2.0, LLC, Providence, RI 02903
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Leah Seward
Nabsys 2.0, LLC, Providence, RI 02903
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Gregory Simelgor
Nabsys 2.0, LLC, Providence, RI 02903
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Nathan K. Weiner
Nabsys 2.0, LLC, Providence, RI 02903
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Barrett Bready
Nabsys 2.0, LLC, Providence, RI 02903
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Abstract

With the advent of routine short-read genome sequencing has come a growing recognition of the importance of long-range, structural information in applications ranging from sequence assembly to the detection of structural variation. Here we describe the Nabsys solid-state detector capable of detecting tags on single molecules of DNA 100s of kilobases in length as they translocate through the detector at a velocity greater than 1 megabase pair per second. Sequence-specific tags are detected with a high signal-to-noise ratio. The physical distance between tags is determined after accounting for viscous drag-induced intramolecular velocity fluctuations. The accurate measurement of the physical distance between tags on each molecule and the ability of the detector to resolve distances between tags of less than 300 base-pairs enables the construction of high-density genome maps.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted May 18, 2017.
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High-Definition Electronic Genome Maps from Single Molecule Data
John S. Oliver, Anthony Catalano, Jennifer R. Davis, Boris S. Grinberg, Timothy E. Hutchins, Michael D. Kaiser, Steve Nurnberg, Jay M. Sage, Leah Seward, Gregory Simelgor, Nathan K. Weiner, Barrett Bready
bioRxiv 139840; doi: https://doi.org/10.1101/139840
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High-Definition Electronic Genome Maps from Single Molecule Data
John S. Oliver, Anthony Catalano, Jennifer R. Davis, Boris S. Grinberg, Timothy E. Hutchins, Michael D. Kaiser, Steve Nurnberg, Jay M. Sage, Leah Seward, Gregory Simelgor, Nathan K. Weiner, Barrett Bready
bioRxiv 139840; doi: https://doi.org/10.1101/139840

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