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Cell dynamics underlying oriented growth of the Drosophila wing imaginal disc

Natalie A. Dye, Marko Popović, Stephanie Spannl, Raphaël Etournay, Dagmar Kainmüller, Eugene W. Myers, Frank Jülicher, Suzanne Eaton
doi: https://doi.org/10.1101/140038
Natalie A. Dye
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01309 Dresden, Germany
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Marko Popović
2Max Planck Institute for the Physics of Complex Systems, Noethnitzer Strasse 38, 01187 Dresden, Germany
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Stephanie Spannl
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01309 Dresden, Germany
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Raphaël Etournay
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01309 Dresden, Germany
3Unité de Génétique et Physiologie de l’Audition, Institut Pasteur, 75015 Paris, France
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Dagmar Kainmüller
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01309 Dresden, Germany
4Janelia Farm Research Campus, 19700 Helix Dr, Ashburn, VA 20147, USA
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Eugene W. Myers
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01309 Dresden, Germany
5Center for Systems Biology Dresden, Pfotenhauerstrasse 108, 01309 Dresden, Germany
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Frank Jülicher
2Max Planck Institute for the Physics of Complex Systems, Noethnitzer Strasse 38, 01187 Dresden, Germany
5Center for Systems Biology Dresden, Pfotenhauerstrasse 108, 01309 Dresden, Germany
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Suzanne Eaton
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01309 Dresden, Germany
6Biotechnologisches Zentrum, Technische Universität Dresden, Tatzberg 47/49, 01309 Dresden, Germany
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ABSTRACT

Quantitative analysis of the dynamic cellular mechanisms shaping the Drosophila wing during its larval growth phase has been limited, impeding our ability to understand how morphogen patterns regulate tissue shape. Such analysis requires imaging explants under conditions that maintain both growth and patterning, as well as methods to quantify how much cellular behaviors change tissue shape. Here, we demonstrate a key requirement for the steroid hormone 20-hydroxyecdysone (20E) in the maintenance of numerous patterning systems in vivo and in explant culture. We find that low concentrations of 20E support prolonged proliferation in explanted wing discs in the absence of insulin, incidentally providing novel insight into the hormonal regulation of imaginal growth. We use 20E-containing media to directly observe growth and apply recently developed methods for quantitatively decomposing tissue shape changes into cellular contributions. We discover that while cell divisions drive tissue expansion along one axis, their contribution to expansion along the orthogonal axis is cancelled by cell rearrangements and cell shape changes. This finding raises the possibility that anisotropic mechanical constraints contribute to growth orientation in the wing disc.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 19, 2017.
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Cell dynamics underlying oriented growth of the Drosophila wing imaginal disc
Natalie A. Dye, Marko Popović, Stephanie Spannl, Raphaël Etournay, Dagmar Kainmüller, Eugene W. Myers, Frank Jülicher, Suzanne Eaton
bioRxiv 140038; doi: https://doi.org/10.1101/140038
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Cell dynamics underlying oriented growth of the Drosophila wing imaginal disc
Natalie A. Dye, Marko Popović, Stephanie Spannl, Raphaël Etournay, Dagmar Kainmüller, Eugene W. Myers, Frank Jülicher, Suzanne Eaton
bioRxiv 140038; doi: https://doi.org/10.1101/140038

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