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A complete electron microscopy volume of the brain of adult Drosophila melanogaster

Zhihao Zheng, J. Scott Lauritzen, Eric Perlman, Camenzind G. Robinson, Matthew Nichols, Daniel Milkie, Omar Torrens, John Price, Corey B. Fisher, Nadiya Sharifi, Steven A. Calle-Schuler, Lucia Kmecova, Iqbal J. Ali, Bill Karsh, Eric T. Trautman, John Bogovic, Philipp Hanslovsky, Gregory S. X. E. Jefferis, Michael Kazhdan, Khaled Khairy, Stephan Saalfeld, Richard D. Fetter, Davi D. Bock
doi: https://doi.org/10.1101/140905
Zhihao Zheng
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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J. Scott Lauritzen
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Eric Perlman
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Camenzind G. Robinson
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Matthew Nichols
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Daniel Milkie
Coleman Technologies, Inc., Newtown Square, PA 19073
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Omar Torrens
Coleman Technologies, Inc., Newtown Square, PA 19073
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John Price
Hudson Price Designs, LLC, Hingham, MA 02043
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Corey B. Fisher
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Nadiya Sharifi
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Steven A. Calle-Schuler
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Lucia Kmecova
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Iqbal J. Ali
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Bill Karsh
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Eric T. Trautman
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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John Bogovic
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Philipp Hanslovsky
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Gregory S. X. E. Jefferis
Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK
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Michael Kazhdan
Department of Computer Science, Johns Hopkins University, Baltimore, MD 21218
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Khaled Khairy
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Stephan Saalfeld
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Richard D. Fetter
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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Davi D. Bock
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
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SUMMARY

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000–neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly. We validated the dataset by tracing brain-spanning circuitry involving the mushroom body (MB), intensively studied for its role in learning. Here we describe the complete set of olfactory inputs to the MB; find a new cell type providing driving input to Kenyon cells (the intrinsic MB neurons); identify neurons postsynaptic to Kenyon cell dendrites; and find that axonal arbors providing input to the MB calyx are more tightly clustered than previously indicated by light-level data. This freely available EM dataset will significantly accelerate Drosophila neuroscience.

  • - A complete adult fruit fly brain was imaged, using electron microscopy (EM)

  • - The EM volume enables brain-spanning mapping of neuronal circuits at the synaptic level

  • - Olfactory projection neurons cluster more tightly in mushroom body calyx than expected from light-level data

  • - The primary postsynaptic targets of Kenyon cells (KCs) in the MB are other KCs, as well as the anterior paired lateral (APL) neuron

  • - A newly discovered cell type, MB-CP2, integrates input from several sensory modalities and provides microglomerular input to KCs in MB calyx

  • - A software pipeline was created in which EM-traced skeletons can be searched for within existing large-scale light microscopy (LM) databases of neuronal morphology, facilitating cell type identification and discovery of relevant genetic driver lines

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted June 13, 2017.
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A complete electron microscopy volume of the brain of adult Drosophila melanogaster
Zhihao Zheng, J. Scott Lauritzen, Eric Perlman, Camenzind G. Robinson, Matthew Nichols, Daniel Milkie, Omar Torrens, John Price, Corey B. Fisher, Nadiya Sharifi, Steven A. Calle-Schuler, Lucia Kmecova, Iqbal J. Ali, Bill Karsh, Eric T. Trautman, John Bogovic, Philipp Hanslovsky, Gregory S. X. E. Jefferis, Michael Kazhdan, Khaled Khairy, Stephan Saalfeld, Richard D. Fetter, Davi D. Bock
bioRxiv 140905; doi: https://doi.org/10.1101/140905
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A complete electron microscopy volume of the brain of adult Drosophila melanogaster
Zhihao Zheng, J. Scott Lauritzen, Eric Perlman, Camenzind G. Robinson, Matthew Nichols, Daniel Milkie, Omar Torrens, John Price, Corey B. Fisher, Nadiya Sharifi, Steven A. Calle-Schuler, Lucia Kmecova, Iqbal J. Ali, Bill Karsh, Eric T. Trautman, John Bogovic, Philipp Hanslovsky, Gregory S. X. E. Jefferis, Michael Kazhdan, Khaled Khairy, Stephan Saalfeld, Richard D. Fetter, Davi D. Bock
bioRxiv 140905; doi: https://doi.org/10.1101/140905

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