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The Proteolytic Landscape of an Arabidopsis Separase-Deficient Mutant Reveals Novel Substrates Associated With Plant Development

Chen Liu, Simon Stael, Kris Gevaert, Frank Van Breusegem, Peter V Bozhkov, Panagiotis N Moschou
doi: https://doi.org/10.1101/140962
Chen Liu
1Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, Sweden
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Simon Stael
2Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium
3Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
4VIB-UGent Center for Medical Biotechnology, 9000 Ghent, Belgium
5Department of Biochemistry, Ghent University, 9000 Ghent, Belgium
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Kris Gevaert
4VIB-UGent Center for Medical Biotechnology, 9000 Ghent, Belgium
5Department of Biochemistry, Ghent University, 9000 Ghent, Belgium
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Frank Van Breusegem
2Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium
3Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
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Peter V Bozhkov
6Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, Sweden
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Panagiotis N Moschou
1Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, Sweden
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Abstract

Digestive proteolysis executed by the proteasome plays an important role in plant development. Yet, the role of limited proteolysis in this process is still obscured due to the absence of studies. Previously, we showed that limited proteolysis by the caspase-related protease separase (EXTRA SPINDLE POLES [ESP]) modulates development in plants through the cleavage of unknown substrates. Here we used a modified version of the positional proteomics method COmbined FRActional DIagonal Chromatography (COFRADIC) to survey the proteolytic landscape of wild-type and separase mutant RADIALLY SWOLLEN 4 (rsw4) root tip cells, as an attempt to identify targets of separase. We have discovered that proteins involved in the establishment of pH homeostasis and sensing, and lipid signalling in wild-type cells, suggesting novel potential roles for separase. We also observed significant accumulation of the protease PRX34 in rsw4 which negatively impacts growth. Furthermore, we observed an increased acetylation of N-termini of rsw4 proteins which usually comprise degrons identified by the ubiquitin-proteasome system, suggesting that separase intersects with additional proteolytic networks. Our results hint to potential pathways by which separase could regulate development suggesting also novel proteolytic functions.

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Posted May 24, 2017.
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The Proteolytic Landscape of an Arabidopsis Separase-Deficient Mutant Reveals Novel Substrates Associated With Plant Development
Chen Liu, Simon Stael, Kris Gevaert, Frank Van Breusegem, Peter V Bozhkov, Panagiotis N Moschou
bioRxiv 140962; doi: https://doi.org/10.1101/140962
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The Proteolytic Landscape of an Arabidopsis Separase-Deficient Mutant Reveals Novel Substrates Associated With Plant Development
Chen Liu, Simon Stael, Kris Gevaert, Frank Van Breusegem, Peter V Bozhkov, Panagiotis N Moschou
bioRxiv 140962; doi: https://doi.org/10.1101/140962

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