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An Efficient CRISPR protocol for generating Conditional and Knock-in mice using long single-stranded DNA donors

Hiromi Miura, Rolen M. Quadros, View ORCID ProfileChannabasavaiah B. Gurumurthy, Masato Ohtsuka
doi: https://doi.org/10.1101/141424
Hiromi Miura
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa 259–1193, Japan
2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Kanagawa 259-1193, Japan
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Rolen M. Quadros
3Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA
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Channabasavaiah B. Gurumurthy
3Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA
4Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA
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  • ORCID record for Channabasavaiah B. Gurumurthy
  • For correspondence: masato@is.icc.u-tokai.ac.jp cgurumurthy@unmc.edu
Masato Ohtsuka
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa 259–1193, Japan
2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Kanagawa 259-1193, Japan
5The Institute of Medical Sciences, Tokai University, Kanagawa 259–1193, Japan
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  • For correspondence: masato@is.icc.u-tokai.ac.jp cgurumurthy@unmc.edu
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Article Information

doi 
https://doi.org/10.1101/141424
History 
  • May 23, 2017.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Hiromi Miura1,26,
  2. Rolen M. Quadros36,
  3. Channabasavaiah B. Gurumurthy3,4,7 and
  4. Masato Ohtsuka1,2,5,7
  1. 1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa 259–1193, Japan
  2. 2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Kanagawa 259-1193, Japan
  3. 3Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA
  4. 4Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA
  5. 5The Institute of Medical Sciences, Tokai University, Kanagawa 259–1193, Japan
  1. ↵7Corresponding authors: masato{at}is.icc.u-tokai.ac.jp and cgurumurthy{at}unmc.edu,
  1. ↵6 Contributed equally

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Posted May 23, 2017.
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An Efficient CRISPR protocol for generating Conditional and Knock-in mice using long single-stranded DNA donors
Hiromi Miura, Rolen M. Quadros, Channabasavaiah B. Gurumurthy, Masato Ohtsuka
bioRxiv 141424; doi: https://doi.org/10.1101/141424
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An Efficient CRISPR protocol for generating Conditional and Knock-in mice using long single-stranded DNA donors
Hiromi Miura, Rolen M. Quadros, Channabasavaiah B. Gurumurthy, Masato Ohtsuka
bioRxiv 141424; doi: https://doi.org/10.1101/141424

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