Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
Confirmatory Results

An Efficient CRISPR protocol for generating Conditional and Knock-in mice using long single-stranded DNA donors

Hiromi Miura, Rolen M. Quadros, View ORCID ProfileChannabasavaiah B. Gurumurthy, Masato Ohtsuka
doi: https://doi.org/10.1101/141424
Hiromi Miura
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa 259–1193, Japan
2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Kanagawa 259-1193, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rolen M. Quadros
3Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Channabasavaiah B. Gurumurthy
3Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA
4Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Channabasavaiah B. Gurumurthy
  • For correspondence: masato@is.icc.u-tokai.ac.jp cgurumurthy@unmc.edu
Masato Ohtsuka
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa 259–1193, Japan
2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Kanagawa 259-1193, Japan
5The Institute of Medical Sciences, Tokai University, Kanagawa 259–1193, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: masato@is.icc.u-tokai.ac.jp cgurumurthy@unmc.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Article usage

Article usage: January 2018 to March 2023

AbstractFullPdf
Jan 20183320164
Feb 20181030111
Mar 2018105097
Apr 2018960135
May 2018860128
Jun 2018770110
Jul 2018580349
Aug 201882088
Sep 2018820129
Oct 2018710135
Nov 2018820102
Dec 201854078
Jan 201953069
Feb 201934079
Mar 201969667
Apr 201928474
May 2019301453
Jun 2019261253
Jul 2019241963
Aug 2019271134
Oct 2019291128
Nov 2019271816
Dec 201950919
Jan 2020451430
Feb 2020351531
Mar 2020361525
May 2020421130
Jun 2020241220
Jul 202022519
Aug 202015843
Sep 2020311111
Oct 202036912
Nov 2020411233
Dec 2020271022
Jan 2021321530
Feb 2021211225
Mar 2021201126
Apr 2021181220
May 2021142016
Jun 2021151614
Jul 2021111015
Aug 2021231018
Sep 202112921
Oct 20218422
Nov 20217613
Dec 20211345
Jan 20222009
Feb 202216012
Mar 202210116
Apr 20228011
May 202212423
Jun 20228820
Jul 20226849
Aug 202230911
Sep 202223516
Oct 202244328
Nov 202223417
Dec 2022537
Jan 2023211226
Feb 2023101011
Mar 2023141315
Back to top
PreviousNext
Posted May 23, 2017.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
An Efficient CRISPR protocol for generating Conditional and Knock-in mice using long single-stranded DNA donors
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
An Efficient CRISPR protocol for generating Conditional and Knock-in mice using long single-stranded DNA donors
Hiromi Miura, Rolen M. Quadros, Channabasavaiah B. Gurumurthy, Masato Ohtsuka
bioRxiv 141424; doi: https://doi.org/10.1101/141424
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
An Efficient CRISPR protocol for generating Conditional and Knock-in mice using long single-stranded DNA donors
Hiromi Miura, Rolen M. Quadros, Channabasavaiah B. Gurumurthy, Masato Ohtsuka
bioRxiv 141424; doi: https://doi.org/10.1101/141424

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4237)
  • Biochemistry (9147)
  • Bioengineering (6786)
  • Bioinformatics (24025)
  • Biophysics (12137)
  • Cancer Biology (9545)
  • Cell Biology (13795)
  • Clinical Trials (138)
  • Developmental Biology (7642)
  • Ecology (11716)
  • Epidemiology (2066)
  • Evolutionary Biology (15518)
  • Genetics (10650)
  • Genomics (14332)
  • Immunology (9493)
  • Microbiology (22858)
  • Molecular Biology (9103)
  • Neuroscience (49032)
  • Paleontology (355)
  • Pathology (1484)
  • Pharmacology and Toxicology (2572)
  • Physiology (3849)
  • Plant Biology (8338)
  • Scientific Communication and Education (1472)
  • Synthetic Biology (2296)
  • Systems Biology (6196)
  • Zoology (1302)