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The peach RGF/GLV signalling peptide pCTG134 is involved in a regulatory circuit that sustains auxin and ethylene actions

Nicola Busatto, Umberto Salvagnin, View ORCID ProfileFrancesca Resentini, Silvia Quaresimin, View ORCID ProfileLorella Navazio, Oriano Marin, View ORCID ProfileMaria Pellegrini, View ORCID ProfileFabrizio Costa, Dale F. Mierke, View ORCID ProfileLivio Trainotti
doi: https://doi.org/10.1101/141705
Nicola Busatto
1Department of Biology, University of Padova, Viale G. Colombo 3, I-35131 Padova, Italy
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Umberto Salvagnin
1Department of Biology, University of Padova, Viale G. Colombo 3, I-35131 Padova, Italy
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Francesca Resentini
1Department of Biology, University of Padova, Viale G. Colombo 3, I-35131 Padova, Italy
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Silvia Quaresimin
1Department of Biology, University of Padova, Viale G. Colombo 3, I-35131 Padova, Italy
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Lorella Navazio
1Department of Biology, University of Padova, Viale G. Colombo 3, I-35131 Padova, Italy
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Oriano Marin
2Department of Biomedical Sciences, University of Padova, Viale G. Colombo 3, I-35131 Padova, Italy
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Maria Pellegrini
3Chemistry Department, Dartmouth College, 03755 Hanover, NH, United States of America
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Fabrizio Costa
4Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38010 San Michele all’Adige, Trento, Italy
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Dale F. Mierke
3Chemistry Department, Dartmouth College, 03755 Hanover, NH, United States of America
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Livio Trainotti
1Department of Biology, University of Padova, Viale G. Colombo 3, I-35131 Padova, Italy
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Abstract

Peach is a climacteric species whose ripening is regulated by the plant hormone ethylene. A crosstalk mechanism with auxin is necessary to support climacteric ethylene synthesis. The homeostasis control of auxin is regulated also by the activity of peptide hormones (PHs), acting both as short and long distant ligands. In this work, we investigated the role of CTG134, a peach gene encoding a GOLVEN-like PH isolated in mesocarp at the onset of ripening.

In peach fruit, CTG134 was expressed during the climacteric transition and its mRNA level was induced by auxin and 1-methylcyclopropene (1-MCP) treatments, whereas it was minimally affected by ethylene. To better elucidate its function, CTG134 was overexpressed in Arabidopsis and tobacco, which showed abnormal root hair growth, similar to wild-type plants treated with a synthetic form of the peptide. Molecular surveys demonstrated an impaired hormonal crosstalk, resulting in a re-modulated expression of a set of genes involved in both ethylene and auxin domains. In addition, the promoter of pCTG134 fused with GUS reporter highlighted gene activity in plant organs in which the auxin-ethylene interplay is known to occur. These data support the role of pCTG134 as mediator in an auxin-ethylene regulatory circuit.

Highlight The role of the peach RGF/GLV peptide during root hair formation in Arabidopsis and tobacco supports its involvement in a cross-hormonal auxin-ethylene regulatory circuit.

Footnotes

  • Email addresses Nicola Busatto: nicola.busatto{at}fmach.it, Umberto Salvagnin: umberto.salvagnin{at}unipd.it, Francesca Resentini: francesca.resentini{at}gmail.com, Silvia Quaresimin: silvia.quaresimin{at}unipd.it, Lorella Navazio: lorella.navazio{at}unipd.it, Oriano Marin: oriano.marin{at}unipd.it, Maria Pellegrini: Maria.Pellegrini{at}dartmouth.edu, Fabrizio Costa: fabrizio.costa{at}fmach.it, Dale F. Mierke: Dale.F.Mierke{at}dartmouth.edu, Livio Trainotti: telephone: +39 049 8276292; email: livio.trainotti{at}unipd.it

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 26, 2017.
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The peach RGF/GLV signalling peptide pCTG134 is involved in a regulatory circuit that sustains auxin and ethylene actions
Nicola Busatto, Umberto Salvagnin, Francesca Resentini, Silvia Quaresimin, Lorella Navazio, Oriano Marin, Maria Pellegrini, Fabrizio Costa, Dale F. Mierke, Livio Trainotti
bioRxiv 141705; doi: https://doi.org/10.1101/141705
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The peach RGF/GLV signalling peptide pCTG134 is involved in a regulatory circuit that sustains auxin and ethylene actions
Nicola Busatto, Umberto Salvagnin, Francesca Resentini, Silvia Quaresimin, Lorella Navazio, Oriano Marin, Maria Pellegrini, Fabrizio Costa, Dale F. Mierke, Livio Trainotti
bioRxiv 141705; doi: https://doi.org/10.1101/141705

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